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IRF1 (NM_002198) Human Over-expression Lysate

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SKU
LC419474
Lyophilized IRF1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC203500
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002198, NP_002189
Synonyms IRF-1; MAR
Predicted MW 36.5 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002189
Locus ID 3659
Cytogenetics 5q31.1
Protein Families WB
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Other "IRF1" proteins (3)
SKU Description Size Price
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TP303500 Recombinant protein of human interferon regulatory factor 1 (IRF1), 20 µg 20 ug
$867.00

file-alt Citations

Interferon regulatory factor 1-Rab27a regulated extracellular vesicles promote liver ischemia/reperfusion injury
Yang, Du, Goswami et al
Hepatology (2017)
AI Summary: OriGene provided the human IRF-1 expression plasmid and backbone plasmid, which were used in this study to investigate the regulatory role of IRF-1 in Rab27a expression and extracellular vesicle secretion during liver ischemia/reperfusion injury.
Snippet: purchased from American Type Culture Collection (ATCC;Rockville, MD, USA). Human IRF-1 expression plasmid and backbone plasmid were ordered from Origene (Rockville, MD). β-galactosidase expression (β-Gal) plasmid was purchased from Promega (Madison
Immune mediators regulate CFTR expression through a bifunctional airway-selective enhancer
Zhang, Leir, Harris
Mol. Cell. Biol. (2013) 33 (15), 2843-53 DOI: 10.1128/MCB.00003-13
Cited by 1
AI Summary: OriGene’s IRF1 (SC118744) and IRF2 cDNA clones were used in this study to overexpress these transcription factors in airway epithelial cells, enabling the authors to investigate their roles in regulating CFTR gene expression.
Snippet: RNAiMAX (Life Technologies, Grand Island, NY) per the manufacturer's protocol. Cells 130 were harvested 72 hrs after transfection to isolate whole cell lysate and total RNA. 131 cDNA clones of IRF1 (SC118744, OriGene Technologies Rockville, MD), IRF2 132
Showing 1-2 of 2 papers.
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