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LAMP2 (NM_002294) Human Over-expression Lysate

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SKU
LY419414
Transient overexpression lysate of lysosomal-associated membrane protein 2 (LAMP2), transcript variant A (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221216
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002294, NP_002285
Synonyms CD107b; DND; LAMP-2; LAMPB; LGP-96; LGP110
Predicted MW 44.96 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002285
Locus ID 3920
Cytogenetics Xq24
Protein Families WB
Protein Pathways Lysosome
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Other "LAMP2" proteins (8)
SKU Description Size Price
PH300456 LAMP2 MS Standard C13 and N15-labeled recombinant protein (NP_054701) 10 ug
$3,360.00
PH321216 LAMP2 MS Standard C13 and N15-labeled recombinant protein (NP_002285) 10 ug
$3,360.00
PH325644 LAMP2 MS Standard C13 and N15-labeled recombinant protein (NP_001116078) 10 ug
$3,360.00
LC419414 Lyophilized LAMP2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP300456 Recombinant protein of human lysosomal-associated membrane protein 2 (LAMP2), transcript variant B, 20 µg 20 ug
$867.00
TP321216 Recombinant protein of human lysosomal-associated membrane protein 2 (LAMP2), transcript variant A, 20 µg 20 ug
$867.00
TP325644 Purified recombinant protein of Homo sapiens lysosomal-associated membrane protein 2 (LAMP2), transcript variant C, 20 µg 20 ug
$867.00
TP720402 Recombinant protein of human lysosomal-associated membrane protein 2 (LAMP2), transcript variant C 10 ug
$235.00

file-alt Citations

Chronic acidosis in the tumour microenvironment selects for overexpression of LAMP2 in the plasma membrane
Damaghi, Tafreshi, Lloyd et al
Nat Commun (2015) 6, 8752 DOI: 10.1038/ncomms9752
Cited by 185
AI Summary: OriGene products, including Trilencer-27 LAMP2 siRNA, LAMP2 shRNA plasmid (TR311794), and LAMP2 overexpression plasmid (RC221216), were used in this study to manipulate LAMP2 expression in breast cancer cell lines, enabling the authors to investigate the role of LAMP2 in cellular adaptation to chronic acidosis.
Snippet: … LAMP2 siRNAs (Trilencer-27: 27mer siRNA, Origene) were transfected using Lipofectamine RNAiMAX (Invitrogen # 13778030) according to the manufacturer’s protocol. To establish stable cell lines, the MCF-7 and MDA-MB-231 cells were infected with Plasmids expressing shRNA to LAMP2 (TR311794, Origene), LAMP2 overexpressing (RC221216, Origene) and red fluorescent protein or GFP expressing using Fugene 6 (Promega) at an early passage and were selected using 2 μg ml−1 puromycin (Sigma)
FigureFigureFigure
Showing 1-1 of 1 papers.
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