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PDE2A (NM_002599) Human Over-expression Lysate

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SKU
LY419215
Transient overexpression lysate of phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC207219
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002599, NP_002590
Synonyms CGS-PDE; cGSPDE; PDE2A1; PED2A4
Predicted MW 105.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002590
Locus ID 5138
Cytogenetics 11q13.4
Protein Families WB
Protein Pathways Progesterone-mediated oocyte maturation, Purine metabolism
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Resources
Other "PDE2A" proteins (1)
SKU Description Size Price
LC419215 Lyophilized PDE2A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Optical Mapping of cAMP Signaling at the Nanometer Scale
Bock, Annibale, Konrad et al
Cell (2020) 182 (6), 1519-1530.e17 DOI: 10.1016/j.cell.2020.07.035
Cited by 193
AI Summary: OriGene contributed to this study by providing the cDNA encoding PDE2A (NM_002599), which was used to investigate the role of phosphodiesterases in cAMP signaling compartmentalization.
Snippet: Skip to article;The cDNA encoding PDE2A (NM_002599) was purchased from OriGene, Rockville, MD, USA. The AKAR4 plasmid (Depry et al., 2011)was a kind gift of Dr. Jin Zhang (UC San Diego, USA) and a plasmid encoding the IRES2 sequence was kindly provided by Dr. GaryLewin (MDC Berlin, Germany)
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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