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PDE2A (NM_002599) Human Over-expression Lysate

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SKU
LC419215
Lyophilized PDE2A HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC207219
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002599, NP_002590
Synonyms CGS-PDE; cGSPDE; PDE2A1; PED2A4
Predicted MW 105.6 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_002590
Locus ID 5138
Cytogenetics 11q13.4
Protein Families WB
Protein Pathways Progesterone-mediated oocyte maturation, Purine metabolism
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Other "PDE2A" proteins (1)
SKU Description Size Price
LY419215 Transient overexpression lysate of phosphodiesterase 2A, cGMP-stimulated (PDE2A), transcript variant 1 (as WB positive control) 100 ug
$436.00

file-alt Citations

Optical Mapping of cAMP Signaling at the Nanometer Scale
Bock, Annibale, Konrad et al
Cell (2020) 182 (6), 1519-1530.e17 DOI: 10.1016/j.cell.2020.07.035
Cited by 193
AI Summary: OriGene contributed to this study by providing the cDNA encoding PDE2A (NM_002599), which was used to investigate the role of phosphodiesterases in cAMP signaling compartmentalization.
Snippet: Skip to article;The cDNA encoding PDE2A (NM_002599) was purchased from OriGene, Rockville, MD, USA. The AKAR4 plasmid (Depry et al., 2011)was a kind gift of Dr. Jin Zhang (UC San Diego, USA) and a plasmid encoding the IRES2 sequence was kindly provided by Dr. GaryLewin (MDC Berlin, Germany)
Showing 1-1 of 1 papers.
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