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RAC2 (NM_002872) Human Over-expression Lysate

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SKU
LY419051
Transient overexpression lysate of ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2) (RAC2) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC200304
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_002872, NP_002863
Synonyms EN-7; Gx; HSPC022; IMD73A; IMD73B; IMD73C; p21-Rac2
Predicted MW 2.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_002863
Locus ID 5880
Cytogenetics 22q13.1
Protein Families WB
Protein Pathways Adherens junction, Axon guidance, B cell receptor signaling pathway, Chemokine signaling pathway, Colorectal cancer, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Leukocyte transendothelial migration, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Pancreatic cancer, Pathways in cancer, Regulation of actin cytoskeleton, VEGF signaling pathway, Viral myocarditis, Wnt signaling pathway
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Documents
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Other "RAC2" proteins (1)
SKU Description Size Price
LC419051 Lyophilized RAC2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Identification of the catalytic site of phospholipase D2 (PLD2) newly described guanine nucleotide exchange factor activity
Mahankali, Henkels, Alter et al
J. Biol. Chem. (2012) 287 (49), 41417-31 DOI: 10.1074/jbc.M112.383596
Cited by 1
AI Summary: In this study, the OriGene Vav-1 protein was used as a positive control to validate guanine nucleotide exchange factor (GEF) activity assays investigating the catalytic site of PLD2.
Snippet: or mutant PXPH (purified). As a positive control we used a well characterized Rac2 GEF, Vav-1, purchased from OriGene (Rockville, MD). Immunoprecipitation, SDS-PAGE, and Western Blot Analyses. To confirm the presence
Showing 1-1 of 1 papers.
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