TRIP13 (NM_004237) Human Over-expression Lysate
SKU
LY418122
Transient overexpression lysate of thyroid hormone receptor interactor 13 (TRIP13), transcript variant 1 (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC201285 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_004237, NP_004228 |
| Synonyms | 16E1BP; MVA3; OOMD9 |
| Predicted MW | 48.6 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_004228 |
| Locus ID | 9319 |
| Cytogenetics | 5p15.33 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
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Resources
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Other "TRIP13" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC418122 | Lyophilized TRIP13 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
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Citations
TRIP13 promotes error-prone nonhomologous end joining and induces chemoresistance in head and neck cancer
Banerjee, Russo, Liu et al
Nat Commun (2014) 5, 4527 DOI: 10.1038/ncomms5527
Banerjee, Russo, Liu et al
Nat Commun (2014) 5, 4527 DOI: 10.1038/ncomms5527
Cited by 156
AI Summary: OriGene's pCMV6-Flag-TRIP13 and pCMV6-Flag control vector were used to overexpress TRIP13 in UM-SCC-1 cells, enabling the study of TRIP13's role in promoting error-prone DNA repair and chemoresistance in head and neck cancer.
Snippet: TRIP13 was downregulated with TRIP13 siRNA (Dharmacon, Lafayette, CO, USA;sequence of siRNA used is listed in Supplementary Fig. 1C). For overexpression of TRIP13, UM-SCC-1 cells were transfected with pCMV6-Flag-TRIP13 or pCMV6-Flag control vector (Origene, Rockville, MD, USA). For inducible TRIP13 downregulation, unique microRNA-30-based doxycycline-inducible Expression Arrest TRIPZ Lentiviral shRNAmir constructs were purchased from Thermo Scientific Open Biosystem
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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