RAB3D (NM_004283) Human Over-expression Lysate
SKU
LY418099
Transient overexpression lysate of RAB3D, member RAS oncogene family (RAB3D) (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC202236 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_004283, NP_004274 |
| Synonyms | D2-2; GOV; RAB16; RAD3D |
| Predicted MW | 24.3 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_004274 |
| Locus ID | 9545 |
| Cytogenetics | 19p13.2 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "RAB3D" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC418099 | Lyophilized RAB3D HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
Citations
Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers
Frick, Sellier, Mackenzie et al
Acta Neuropathol Commun (2018) 6 (1), 72 DOI: 10.1186/s40478-018-0579-0
Frick, Sellier, Mackenzie et al
Acta Neuropathol Commun (2018) 6 (1), 72 DOI: 10.1186/s40478-018-0579-0
AI Summary: OriGene products, specifically pCMV6 plasmids containing C-terminally myc-DDK-tagged cDNAs for human and mouse C9orf72 isoforms, as well as FLAG-tagged human SMCR8 and Rab proteins, were used for expression studies to investigate C9orf72 protein localization, interactions, and levels in this study.
Snippet: pCMV6 containing C-terminally myc-DDK-tagged cDNAs of human C9orf72 variant 1 (NM_145005), human C9orf72 variant 2 (NM_018325), mouse C9orf72 encoding for isoform 1 (NM_001081343), mouse C9orf72 encoding for isoform 2 (BC026738), C-terminally FLAG-tagged human cDNAs of SMCR8, and Rab proteins were purchased from OriGene. cDNAs for C9orf72 were subcloned into pcDNA5/FRT/TO (Invitrogen) with or without myc-DDK-tag. Optimized cDNA for human N-terminally HA-tagged C9orf72 variant 1 cloned in pcDNA3 was described previously [45] and is available through Addgene
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
