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RAB3D (NM_004283) Human Over-expression Lysate

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SKU
LC418099
Lyophilized RAB3D HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202236
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004283, NP_004274
Synonyms D2-2; GOV; RAB16; RAD3D
Predicted MW 24.3 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_004274
Locus ID 9545
Cytogenetics 19p13.2
Protein Families WB
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Other "RAB3D" proteins (1)
SKU Description Size Price
LY418099 Transient overexpression lysate of RAB3D, member RAS oncogene family (RAB3D) (as WB positive control) 100 ug
$436.00

file-alt Citations

Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers
Frick, Sellier, Mackenzie et al
Acta Neuropathol Commun (2018) 6 (1), 72 DOI: 10.1186/s40478-018-0579-0
AI Summary: OriGene products, specifically pCMV6 plasmids containing C-terminally myc-DDK-tagged cDNAs for human and mouse C9orf72 isoforms, as well as FLAG-tagged human SMCR8 and Rab proteins, were used for expression studies to investigate C9orf72 protein localization, interactions, and levels in this study.
Snippet: pCMV6 containing C-terminally myc-DDK-tagged cDNAs of human C9orf72 variant 1 (NM_145005), human C9orf72 variant 2 (NM_018325), mouse C9orf72 encoding for isoform 1 (NM_001081343), mouse C9orf72 encoding for isoform 2 (BC026738), C-terminally FLAG-tagged human cDNAs of SMCR8, and Rab proteins were purchased from OriGene. cDNAs for C9orf72 were subcloned into pcDNA5/FRT/TO (Invitrogen) with or without myc-DDK-tag. Optimized cDNA for human N-terminally HA-tagged C9orf72 variant 1 cloned in pcDNA3 was described previously [45] and is available through Addgene
FigureFigureFigure
Showing 1-1 of 1 papers.
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