Her2 (ERBB2) (NM_004448) Human Over-expression Lysate
CAT#: LY417979
Transient overexpression lysate of v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1
Size: 20 ug
View other "Her2" proteins (18)
Special Offer: Get a 20% discount on this product. Use code: "OEL20".
Specifications
Product Data | |
Species | Human |
Applications | WB |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC212583 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Accession Number | NM_004448, NP_004439 |
Other Names | CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1 |
Predicted MW | 137.7 kDa |
Components | 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer 1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT) |
Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
Reference Data | |
RefSeq | NP_004439 |
Locus ID | 2064 |
Cytogenetics | 17q12 |
Protein Families | WB |
Protein Pathways | Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Recombinant Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC417979 | ERBB2 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 206.00 |
|
LC423666 | ERBB2 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 206.00 |
|
LY423666 | Transient overexpression lysate of v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 2 |
USD 665.00 |
|
PH312583 | ERBB2 MS Standard C13 and N15-labeled recombinant protein (NP_004439) |
USD 3,255.00 |
|
PH322909 | ERBB2 MS Standard C13 and N15-labeled recombinant protein (NP_001005862) |
USD 3,255.00 |
|
TP312583 | Recombinant protein of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, 20 µg |
USD 867.00 |
|
TP322909 | Recombinant protein of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 2, 20 µg |
USD 867.00 |
|
TP700105 | Recombinant protein of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, extracellular domain, 20 µg |
USD 867.00 |
|
TP710004 | Recombinant protein of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2,neuro/glioblastoma derived oncogene homolog (avian) (ERBB2),residues 676-1255aa,with C-terminal DDK tag,expressed in sf9s, 20ug |
USD 515.00 |
|
TP710032 | Recombinant protein of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), full length, with C-terminal DDK tag, expressed in sf9 cells. |
USD 515.00 |
|
TP710036 | Recombinant protein of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), residues 23-652aa, with C-terminal DDK tag, expressed in sf9 cells. |
USD 515.00 |
|
TP721313 | Human Her2 Protein (C-Fc) |
USD 258.00 |
|
TP721314 | Human Her2 Protein (C-His) |
USD 258.00 |
|
TP721315 | Human HER2 Protein (C-His-Avi) |
USD 258.00 |
|
TP721316 | Biotinylated Human HER2 Protein (C-His-Avi) |
USD 366.00 |
|
TP721317 | PE Conjugated Human Her2 Protein (C-His) |
USD 366.00 |
|
TP721318 | APC Conjugated Human Her2 Protein (C-His) |
USD 366.00 |
|
TP790102 | Purified recombinant protein of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, full length, with C-terminal DDK tag,expressed in CHO cells; |
USD 871.00 |
{0} Product Review(s)
Be the first one to submit a review