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Her2 (ERBB2) (NM_004448) Human Over-expression Lysate

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SKU
LY417979
Transient overexpression lysate of v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1 (as WB positive control)
  $665.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC212583
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_004448, NP_004439
Synonyms CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1
Predicted MW 137.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_004439
Locus ID 2064
Cytogenetics 17q12
Protein Families WB
Protein Pathways Adherens junction, Bladder cancer, Calcium signaling pathway, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer
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Other "Her2" proteins (18)
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PH312583 ERBB2 MS Standard C13 and N15-labeled recombinant protein (NP_004439) 10 ug
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PH322909 ERBB2 MS Standard C13 and N15-labeled recombinant protein (NP_001005862) 10 ug
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LC417979 Lyophilized ERBB2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LC423666 Lyophilized ERBB2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
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LY423666 Transient overexpression lysate of v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 2 (as WB positive control) 100 ug
$665.00
TP312583 Recombinant protein of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, 20 µg 20 ug
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TP322909 Recombinant protein of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 2, 20 µg 20 ug
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TP700105 Recombinant protein of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, extracellular domain, 20 µg 20 ug
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TP710004 Recombinant protein of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2,neuro/glioblastoma derived oncogene homolog (avian) (ERBB2),residues 676-1255aa,with C-terminal DDK tag,expressed in sf9s, 20ug 20 ug
$540.00
TP710032 Recombinant protein of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), full length, with C-terminal DDK tag, expressed in sf9 cells. 20 ug
$540.00
TP710036 Recombinant protein of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), residues 23-652aa, with C-terminal DDK tag, expressed in sf9 cells. 20 ug
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TP721313 Human Her2 Protein (C-Fc) 25 ug
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TP721314 Human Her2 Protein (C-His) 25 ug
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TP721315 Human HER2 Protein (C-His-Avi) 25 ug
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TP721316 Biotinylated Human HER2 Protein (C-His-Avi) 25 ug
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TP721317 PE Conjugated Human Her2 Protein (C-His) 25 ug
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TP721318 APC Conjugated Human Her2 Protein (C-His) 25 ug
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TP790102 Purified recombinant protein of Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 1, full length, with C-terminal DDK tag,expressed in CHO cells; 50 ug
$900.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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