PLK1 (NM_005030) Human Over-expression Lysate
SKU
LY417592
Transient overexpression lysate of polo-like kinase 1 (Drosophila) (PLK1) (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC201795 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_005030, NP_005021 |
| Synonyms | PLK; STPK13 |
| Predicted MW | 68.1 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_005021 |
| Locus ID | 5347 |
| Cytogenetics | 16p12.2 |
| Protein Families | WB |
| Protein Pathways | Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "PLK1" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH301795 | PLK1 MS Standard C13 and N15-labeled recombinant protein (NP_005021) | 10 ug |
$3,360.00
|
|
| LC417592 | Lyophilized PLK1 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP301795 | Recombinant protein of human polo-like kinase 1 (Drosophila) (PLK1), 20 µg | 20 ug |
$737.00
|
|
Citations
HIV-1 blocks the signaling adaptor MAVS to evade antiviral host defense after sensing of abortive HIV-1 RNA by the host helicase DDX3
Gringhuis, Hertoghs, Kaptein et al
Nat. Immunol. (2016)
Gringhuis, Hertoghs, Kaptein et al
Nat. Immunol. (2016)
AI Summary: OriGene’s Thr210-phosphorylated Flag–PLK1 protein was used in capture ELISA assays to investigate the interaction between PLK1 and MAVS, helping to elucidate how HIV-1 disrupts antiviral signaling in dendritic cells.
Snippet: Association of PLK1 with either wild-type or mutant MAVS was further examined by capture ELISA. 50 ng Thr210-phosphorylated Flag–PLK1 (Origene;see below) was immobilized on anti-PLK1 (4513;Cell Signaling)-coated high-binding 96-well plates and incubated with whole-cell extracts (see above) from immature DCs from donors with either the minor or major genotype for rs7262903 and rs7269320
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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