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PLK1 (NM_005030) Human Over-expression Lysate

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SKU
LC417592
Lyophilized PLK1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201795
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005030, NP_005021
Synonyms PLK; STPK13
Predicted MW 68.1 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_005021
Locus ID 5347
Cytogenetics 16p12.2
Protein Families WB
Protein Pathways Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
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Other "PLK1" proteins (3)
SKU Description Size Price
PH301795 PLK1 MS Standard C13 and N15-labeled recombinant protein (NP_005021) 10 ug
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$436.00
TP301795 Recombinant protein of human polo-like kinase 1 (Drosophila) (PLK1), 20 µg 20 ug
$737.00

file-alt Citations

HIV-1 blocks the signaling adaptor MAVS to evade antiviral host defense after sensing of abortive HIV-1 RNA by the host helicase DDX3
Gringhuis, Hertoghs, Kaptein et al
Nat. Immunol. (2016)
AI Summary: OriGene’s Thr210-phosphorylated Flag–PLK1 protein was used in capture ELISA assays to investigate the interaction between PLK1 and MAVS, helping to elucidate how HIV-1 disrupts antiviral signaling in dendritic cells.
Snippet: Association of PLK1 with either wild-type or mutant MAVS was further examined by capture ELISA. 50 ng Thr210-phosphorylated Flag–PLK1 (Origene;see below) was immobilized on anti-PLK1 (4513;Cell Signaling)-coated high-binding 96-well plates and incubated with whole-cell extracts (see above) from immature DCs from donors with either the minor or major genotype for rs7262903 and rs7269320
Showing 1-1 of 1 papers.
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