OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

POU3F2 (NM_005604) Human Over-expression Lysate

Be the first to review this product
SKU
LY417196
Transient overexpression lysate of POU class 3 homeobox 2 (POU3F2) (as WB positive control)
  $436.00
In Stock*
Bulk/Customize

Frequently Bought Together (3)

TA329222 Rabbit Polyclonal anti-POU3F2 antibody
100 ul
$585.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "POU3F2 or NM_005604"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205330
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_005604, NP_005595
Synonyms brn-2; BRN2; N-Oct3; oct-7; OCT7; OTF-7; OTF7; POUF3
Predicted MW 46.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_005595
Locus ID 5454
Cytogenetics 6q16.1
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "POU3F2" proteins (3)
SKU Description Size Price
PH305330 POU3F2 MS Standard C13 and N15-labeled recombinant protein (NP_005595) 10 ug
$3,360.00
LC417196 Lyophilized POU3F2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP305330 Recombinant protein of human POU class 3 homeobox 2 (POU3F2), 20 µg 20 ug
$867.00

file-alt Citations

SOX10 regulates expression of the SH3-domain kinase binding protein 1 (Sh3kbp1) locus in Schwann cells via an alternative promoter
Hodonsky, Kleinbrink, Charney et al
Mol. Cell. Neurosci. (2012) 49 (2), 85-96 DOI: 10.1016/j.mcn.2011.10.004
Cited by 14
AI Summary: OriGene products contributed to this study by providing the SOX10 cDNA (OriGene, Cat# MR204959) used for ectopic expression experiments in SOX10-negative cells to assess the regulation of Sh3kbp1 expression.
Snippet: Elnitski et al., 2003). Next, we searched for non-coding regions within the alignment that are 100% identical among all six species and at least 6 bp long using the ExactPlus (EP) algorithm (Antonellis et al., 2006). This revealed 12
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products