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Semaphorin 3A (SEMA3A) (NM_006080) Human Over-expression Lysate

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SKU
LY416877
Transient overexpression lysate of sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (SEMA3A) (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC213681
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006080, NP_006071
Synonyms coll-1; COLL1; HH16; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SEMAL; SemD
Predicted MW 88.89 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_006071
Locus ID 10371
Cytogenetics 7q21.11
Protein Families WB
Protein Pathways Axon guidance
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Documents
Resources
Other "Semaphorin 3A" proteins (1)
SKU Description Size Price
LC416877 Lyophilized SEMA3A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

Calcium-Inducible MAPK/AP-1 Signaling Drives Semaphorin 3A Expression in Normal Human Epidermal Keratinocytes
Kamata, Tominaga, Umehara et al
J. Invest. Dermatol. (2020) 140 (7), 1346-1354.e5 DOI: 10.1016/j.jid.2020.01.001
Cited by 26
AI Summary: OriGene products pCMV6-Entry-JunB (Myc-DDK-tagged), pCMV6-Entry-Fra2 (Myc-DDK-tagged), and pCMV6-Entry were used for the overexpression of transcription factors to investigate their effects on Sema3A expression in human epidermal keratinocytes.
Snippet: Overexpression of candidate transcriptional factors. To examine the overexpression of transcription factors on Sema3A expression, we used pCMV6-Entry-JunB (Myc-DDK-tagged), pCMV6-Entry-Fra2 (Myc-DDK-tagged), and pCMV6-Entry (ORIGENE, Rockville, MD)
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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