Semaphorin 3A (SEMA3A) (NM_006080) Human Tagged ORF Clone

SKU RC213681

SEMA3A (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (SEMA3A)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Semaphorin 3A
• Synonyms: coll-1; COLL1; HH16; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SEMAL; SemD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213681 representing NM_006080; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGCTGGTTAACTAGGATTGTCTGTCTTTTCTGGGGAGTATTACTTACAGCAAGAGCAAACTATCAGA; ATGGGAAGAACAATGTGCCAAGGCTGAAATTATCCTACAAAGAAATGTTGGAATCCAACAATGTGATCAC; TTTCAATGGCTTGGCCAACAGCTCCAGTTATCATACCTTCCTTTTGGATGAGGAACGGAGTAGGCTGTAT; GTTGGAGCAAAGGATCACATATTTTCATTCGACCTGGTTAATATCAAGGATTTTCAAAAGATTGTGTGGC; CAGTATCTTACACCAGAAGAGATGAATGCAAGTGGGCTGGAAAAGACATCCTGAAAGAATGTGCTAATTT; CATCAAGGTACTTAAGGCATATAATCAGACTCACTTGTACGCCTGTGGAACGGGGGCTTTTCATCCAATT; TGCACCTACATTGAAATTGGACATCATCCTGAGGACAATATTTTTAAGCTGGAGAACTCACATTTTGAAA; ACGGCCGTGGGAAGAGTCCATATGACCCTAAGCTGCTGACAGCATCCCTTTTAATAGATGGAGAATTATA; CTCTGGAACTGCAGCTGATTTTATGGGGCGAGACTTTGCTATCTTCCGAACTCTTGGGCACCACCACCCA; ATCAGGACAGAGCAGCATGATTCCAGGTGGCTCAATGATCCAAAGTTCATTAGTGCCCACCTCATCTCAG; AGAGTGACAATCCTGAAGATGACAAAGTATACTTTTTCTTCCGTGAAAATGCAATAGATGGAGAACACTC; TGGAAAAGCTACTCACGCTAGAATAGGTCAGATATGCAAGAATGACTTTGGAGGGCACAGAAGTCTGGTG; AATAAATGGACAACATTCCTCAAAGCTCGTCTGATTTGCTCAGTGCCAGGTCCAAATGGCATTGACACTC; ATTTTGATGAACTGCAGGATGTATTCCTAATGAACTTTAAAGATCCTAAAAATCCAGTTGTATATGGAGT; GTTTACGACTTCCAGTAACATTTTCAAGGGATCAGCCGTGTGTATGTATAGCATGAGTGATGTGAGAAGG; GTGTTCCTTGGTCCATATGCCCACAGGGATGGACCCAACTATCAATGGGTGCCTTATCAAGGAAGAGTCC; CCTATCCACGGCCAGGAACTTGTCCCAGCAAAACATTTGGTGGTTTTGACTCTACAAAGGACCTTCCTGA; TGATGTTATAACCTTTGCAAGAAGTCATCCAGCCATGTACAATCCAGTGTTTCCTATGAACAATCGCCCA; ATAGTGATCAAAACGGATGTAAATTATCAATTTACACAAATTGTCGTAGACCGAGTGGATGCAGAAGATG; GACAGTATGATGTTATGTTTATCGGAACAGATGTTGGGACCGTTCTTAAAGTAGTTTCAATTCCTAAGGA; GACTTGGTATGATTTAGAAGAGGTTCTGCTGGAAGAAATGACAGTTTTTCGGGAACCGACTGCTATTTCA; GCAATGGAGCTTTCCACTAAGCAGCAACAACTATATATTGGTTCAACGGCTGGGGTTGCCCAGCTCCCTT; TACACCGGTGTGATATTTACGGGAAAGCGTGTGCTGAGTGTTGCCTCGCCCGAGACCCTTACTGTGCTTG; GGATGGTTCTGCATGTTCTCGCTATTTTCCCACTGCAAAGAGACGCACAAGACGACAAGATATAAGAAAT; GGAGACCCACTGACTCACTGTTCAGACTTACACCATGATAATCACCATGGCCACAGCCCTGAAGAGAGAA; TCATCTATGGTGTAGAGAATAGTAGCACATTTTTGGAATGCAGTCCGAAGTCGCAGAGAGCGCTGGTCTA; TTGGCAATTCCAGAGGCGAAATGAAGAGCGAAAAGAAGAGATCAGAGTGGATGATCATATCATCAGGACA; GATCAAGGCCTTCTGCTACGTAGTCTACAACAGAAGGATTCAGGCAATTACCTCTGCCATGCGGTGGAAC; ATGGGTTCATACAAACTCTTCTTAAGGTAACCCTGGAAGTCATTGACACAGAGCATTTGGAAGAACTTCT; TCATAAAGATGATGATGGAGATGGCTCTAAGACCAAAGAAATGTCCAATAGCATGACACCTAGCCAGAAG; GTCTGGTACAGAGACTTCATGCAGCTCATCAACCACCCCAATCTCAACACGATGGATGAGTTCTGTGAAC; AAGTTTGGAAAAGGGACCGAAAACAACGTCGGCAAAGGCCAGGACATACCCCAGGGAACAGTAACAAATG; GAAGCACTTACAAGAAAATAAGAAAGGTAGAAACAGGAGGACCCACGAATTTGAGAGGGCACCCAGGAGT; GTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213681 representing NM_006080; Red=Cloning site Green=Tags(s); ; MGWLTRIVCLFWGVLLTARANYQNGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLY; VGAKDHIFSFDLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLKAYNQTHLYACGTGAFHPI; CTYIEIGHHPEDNIFKLENSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHP; IRTEQHDSRWLNDPKFISAHLISESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLV; NKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNFKDPKNPVVYGVFTTSSNIFKGSAVCMYSMSDVRR; VFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPMNNRP; IVIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSIPKETWYDLEEVLLEEMTVFREPTAIS; AMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSACSRYFPTAKRRTRRQDIRN; GDPLTHCSDLHHDNHHGHSPEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEERKEEIRVDDHIIRT; DQGLLLRSLQQKDSGNYLCHAVEHGFIQTLLKVTLEVIDTEHLEELLHKDDDGDGSKTKEMSNSMTPSQK; VWYRDFMQLINHPNLNTMDEFCEQVWKRDRKQRRQRPGHTPGNSNKWKHLQENKKGRNRRTHEFERAPRS; V; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006080
• ORF Size: 2313 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006080.1, NP_006071.1
• RefSeq Size: 2530 bp
• RefSeq ORF: 2316 bp
• Locus ID: 10371
• UniProt ID: Q14563
• Cytogenetics: 7q21.11
• Domains: ig, IG, PSI, Sema
• Protein Families: Secreted Protein
• Protein Pathways: Axon guidance
• MW: 88.89 kDa
• Summary: This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]