Human Semaphorin 3A (SEMA3A) (NM_006080) AAV Particle

SKU RC213681A1V

AAV ORF Particle, Serotype AAV-2, SEMA3A (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A (SEMA3A), (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: SEMA3A
• Synonyms: coll-1; COLL1; HH16; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SEMAL; SemD
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213681 representing NM_006080; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGCTGGTTAACTAGGATTGTCTGTCTTTTCTGGGGAGTATTACTTACAGCAAGAGCAAACTATCAGA; ATGGGAAGAACAATGTGCCAAGGCTGAAATTATCCTACAAAGAAATGTTGGAATCCAACAATGTGATCAC; TTTCAATGGCTTGGCCAACAGCTCCAGTTATCATACCTTCCTTTTGGATGAGGAACGGAGTAGGCTGTAT; GTTGGAGCAAAGGATCACATATTTTCATTCGACCTGGTTAATATCAAGGATTTTCAAAAGATTGTGTGGC; CAGTATCTTACACCAGAAGAGATGAATGCAAGTGGGCTGGAAAAGACATCCTGAAAGAATGTGCTAATTT; CATCAAGGTACTTAAGGCATATAATCAGACTCACTTGTACGCCTGTGGAACGGGGGCTTTTCATCCAATT; TGCACCTACATTGAAATTGGACATCATCCTGAGGACAATATTTTTAAGCTGGAGAACTCACATTTTGAAA; ACGGCCGTGGGAAGAGTCCATATGACCCTAAGCTGCTGACAGCATCCCTTTTAATAGATGGAGAATTATA; CTCTGGAACTGCAGCTGATTTTATGGGGCGAGACTTTGCTATCTTCCGAACTCTTGGGCACCACCACCCA; ATCAGGACAGAGCAGCATGATTCCAGGTGGCTCAATGATCCAAAGTTCATTAGTGCCCACCTCATCTCAG; AGAGTGACAATCCTGAAGATGACAAAGTATACTTTTTCTTCCGTGAAAATGCAATAGATGGAGAACACTC; TGGAAAAGCTACTCACGCTAGAATAGGTCAGATATGCAAGAATGACTTTGGAGGGCACAGAAGTCTGGTG; AATAAATGGACAACATTCCTCAAAGCTCGTCTGATTTGCTCAGTGCCAGGTCCAAATGGCATTGACACTC; ATTTTGATGAACTGCAGGATGTATTCCTAATGAACTTTAAAGATCCTAAAAATCCAGTTGTATATGGAGT; GTTTACGACTTCCAGTAACATTTTCAAGGGATCAGCCGTGTGTATGTATAGCATGAGTGATGTGAGAAGG; GTGTTCCTTGGTCCATATGCCCACAGGGATGGACCCAACTATCAATGGGTGCCTTATCAAGGAAGAGTCC; CCTATCCACGGCCAGGAACTTGTCCCAGCAAAACATTTGGTGGTTTTGACTCTACAAAGGACCTTCCTGA; TGATGTTATAACCTTTGCAAGAAGTCATCCAGCCATGTACAATCCAGTGTTTCCTATGAACAATCGCCCA; ATAGTGATCAAAACGGATGTAAATTATCAATTTACACAAATTGTCGTAGACCGAGTGGATGCAGAAGATG; GACAGTATGATGTTATGTTTATCGGAACAGATGTTGGGACCGTTCTTAAAGTAGTTTCAATTCCTAAGGA; GACTTGGTATGATTTAGAAGAGGTTCTGCTGGAAGAAATGACAGTTTTTCGGGAACCGACTGCTATTTCA; GCAATGGAGCTTTCCACTAAGCAGCAACAACTATATATTGGTTCAACGGCTGGGGTTGCCCAGCTCCCTT; TACACCGGTGTGATATTTACGGGAAAGCGTGTGCTGAGTGTTGCCTCGCCCGAGACCCTTACTGTGCTTG; GGATGGTTCTGCATGTTCTCGCTATTTTCCCACTGCAAAGAGACGCACAAGACGACAAGATATAAGAAAT; GGAGACCCACTGACTCACTGTTCAGACTTACACCATGATAATCACCATGGCCACAGCCCTGAAGAGAGAA; TCATCTATGGTGTAGAGAATAGTAGCACATTTTTGGAATGCAGTCCGAAGTCGCAGAGAGCGCTGGTCTA; TTGGCAATTCCAGAGGCGAAATGAAGAGCGAAAAGAAGAGATCAGAGTGGATGATCATATCATCAGGACA; GATCAAGGCCTTCTGCTACGTAGTCTACAACAGAAGGATTCAGGCAATTACCTCTGCCATGCGGTGGAAC; ATGGGTTCATACAAACTCTTCTTAAGGTAACCCTGGAAGTCATTGACACAGAGCATTTGGAAGAACTTCT; TCATAAAGATGATGATGGAGATGGCTCTAAGACCAAAGAAATGTCCAATAGCATGACACCTAGCCAGAAG; GTCTGGTACAGAGACTTCATGCAGCTCATCAACCACCCCAATCTCAACACGATGGATGAGTTCTGTGAAC; AAGTTTGGAAAAGGGACCGAAAACAACGTCGGCAAAGGCCAGGACATACCCCAGGGAACAGTAACAAATG; GAAGCACTTACAAGAAAATAAGAAAGGTAGAAACAGGAGGACCCACGAATTTGAGAGGGCACCCAGGAGT; GTC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213681 representing NM_006080; Red=Cloning site Green=Tags(s); ; MGWLTRIVCLFWGVLLTARANYQNGKNNVPRLKLSYKEMLESNNVITFNGLANSSSYHTFLLDEERSRLY; VGAKDHIFSFDLVNIKDFQKIVWPVSYTRRDECKWAGKDILKECANFIKVLKAYNQTHLYACGTGAFHPI; CTYIEIGHHPEDNIFKLENSHFENGRGKSPYDPKLLTASLLIDGELYSGTAADFMGRDFAIFRTLGHHHP; IRTEQHDSRWLNDPKFISAHLISESDNPEDDKVYFFFRENAIDGEHSGKATHARIGQICKNDFGGHRSLV; NKWTTFLKARLICSVPGPNGIDTHFDELQDVFLMNFKDPKNPVVYGVFTTSSNIFKGSAVCMYSMSDVRR; VFLGPYAHRDGPNYQWVPYQGRVPYPRPGTCPSKTFGGFDSTKDLPDDVITFARSHPAMYNPVFPMNNRP; IVIKTDVNYQFTQIVVDRVDAEDGQYDVMFIGTDVGTVLKVVSIPKETWYDLEEVLLEEMTVFREPTAIS; AMELSTKQQQLYIGSTAGVAQLPLHRCDIYGKACAECCLARDPYCAWDGSACSRYFPTAKRRTRRQDIRN; GDPLTHCSDLHHDNHHGHSPEERIIYGVENSSTFLECSPKSQRALVYWQFQRRNEERKEEIRVDDHIIRT; DQGLLLRSLQQKDSGNYLCHAVEHGFIQTLLKVTLEVIDTEHLEELLHKDDDGDGSKTKEMSNSMTPSQK; VWYRDFMQLINHPNLNTMDEFCEQVWKRDRKQRRQRPGHTPGNSNKWKHLQENKKGRNRRTHEFERAPRS; V; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_006080
• ORF Size: 2313 bp
• Buffer: PBS with 0.001% Pluronic F7098
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_006080.1, NP_006071.1
• RefSeq Size: 2530 bp
• RefSeq ORF: 2316 bp
• Locus ID: 10371
• UniProt ID: Q14563
• Cytogenetics: 7q21.11
• MW: 88.89 kDa