PCP4 (NM_006198) Human Over-expression Lysate
SKU
LY416805
Transient overexpression lysate of Purkinje cell protein 4 (PCP4) (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC203796 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_006198, NP_006189 |
| Synonyms | PEP-19 |
| Predicted MW | 6.8 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_006189 |
| Locus ID | 5121 |
| Cytogenetics | 21q22.2 |
| Protein Families | WB |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "PCP4" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH303796 | PCP4 MS Standard C13 and N15-labeled recombinant protein (NP_006189) | 10 ug |
$3,360.00
|
|
| LC416805 | Lyophilized PCP4 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP303796 | Recombinant protein of human Purkinje cell protein 4 (PCP4), 20 µg | 20 ug |
$867.00
|
|
Citations
PCP4/PEP19 downregulates neurite outgrowth via transcriptional regulation of Ascl1 and NeuroD1 expression in human neuroblastoma M17 cells
Kitazono, Hamada, Yoshimura et al
Lab. Invest. (2020)
Kitazono, Hamada, Yoshimura et al
Lab. Invest. (2020)
Cited by 11
PCP4/PEP19 upregulates aromatase gene expression via CYP19A1 promoter I.1 in human breast cancer SK-BR-3 cells
Honjo, Hamada, Yoshimura et al
Oncotarget (2018) 9 (51), 29619-29633 DOI: 10.18632/oncotarget.25651
Honjo, Hamada, Yoshimura et al
Oncotarget (2018) 9 (51), 29619-29633 DOI: 10.18632/oncotarget.25651
Snippet: eriments, MCF-7 cells were cultured in the medium containing 1 nM E2. Plasmid construction For PCP4/PEP19 overexpression experiments, PCP4 expression vector driven by CMV promoter was purchased from OriGene Technologies (Rockville, MD). Luciferase reporter construct containing human aromatase promoters PI.4 was kindly gifted from Dr. Colin Clyne in Prince Henry’s Institute of Medical Research (
Showing 1-2 of 2 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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