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PCP4 (NM_006198) Human Over-expression Lysate

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SKU
LC416805
Lyophilized PCP4 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC203796
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_006198, NP_006189
Synonyms PEP-19
Predicted MW 6.8 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_006189
Locus ID 5121
Cytogenetics 21q22.2
Protein Families WB
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Other "PCP4" proteins (3)
SKU Description Size Price
PH303796 PCP4 MS Standard C13 and N15-labeled recombinant protein (NP_006189) 10 ug
$3,360.00
LY416805 Transient overexpression lysate of Purkinje cell protein 4 (PCP4) (as WB positive control) 100 ug
$436.00
TP303796 Recombinant protein of human Purkinje cell protein 4 (PCP4), 20 µg 20 ug
$867.00

file-alt Citations

PCP4/PEP19 downregulates neurite outgrowth via transcriptional regulation of Ascl1 and NeuroD1 expression in human neuroblastoma M17 cells
Kitazono, Hamada, Yoshimura et al
Lab. Invest. (2020)
Cited by 11
AI Summary: OriGene’s CMV-driven PCP4/PEP19 cDNA expression vector was used to overexpress PCP4/PEP19 in SH-SY5Y cells, enabling the study of its effects on neuronal differentiation and neurite outgrowth.
Snippet: For PCP4/PEP19 overexpression experiments in SH-SY5Y cells, a CMV-driven expression vector harboring PCP4/PEP19 cDNA (OriGene Technologies, Rockville, MD, USA) was transfected using Lipofectamine 3000 (Thermo Fisher Scientific)
PCP4/PEP19 upregulates aromatase gene expression via CYP19A1 promoter I.1 in human breast cancer SK-BR-3 cells
Honjo, Hamada, Yoshimura et al
Oncotarget (2018) 9 (51), 29619-29633 DOI: 10.18632/oncotarget.25651
Snippet: eriments, MCF-7 cells were cultured in the medium containing 1 nM E2. Plasmid construction For PCP4/PEP19 overexpression experiments, PCP4 expression vector driven by CMV promoter was purchased from OriGene Technologies (Rockville, MD). Luciferase reporter construct containing human aromatase promoters PI.4 was kindly gifted from Dr. Colin Clyne in Prince Henry’s Institute of Medical Research (
FigureFigureFigure
Showing 1-2 of 2 papers.
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