DLL3 (NM_016941) Human Over-expression Lysate
SKU
LY413792
Transient overexpression lysate of delta-like 3 (Drosophila) (DLL3), transcript variant 1 (as WB positive control)
$436.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC200005 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_016941, NP_058637 |
| Synonyms | SCDO1 |
| Predicted MW | 64.6 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_058637 |
| Locus ID | 10683 |
| Cytogenetics | 19q13.2 |
| Protein Families | WB |
| Protein Pathways | Notch signaling pathway |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "DLL3" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC413792 | Lyophilized DLL3 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
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Citations
Near infrared photoimmunotherapy targeting DLL3 for small cell lung cancer
Isobe, Sato, Nishinaga et al
EBioMedicine (2020) 52, 102632 DOI: 10.1016/j.ebiom.2020.102632
Isobe, Sato, Nishinaga et al
EBioMedicine (2020) 52, 102632 DOI: 10.1016/j.ebiom.2020.102632
Cited by 73
AI Summary: OriGene's DLL3-GFP lentiviral particles were used to generate DLL3-expressing cell lines, which were essential for evaluating the efficacy of DLL3-targeted near infrared photoimmunotherapy in this study.
Snippet: ein (GFP), and luciferase-expressing SBC5 and 3T3 cell lines were established by transfecting RediFect Red-FLuc lentiviral particles (PerkinElmer, Waltham, MA, USA) and DLL3-GFP lentiviral particles (OriGene, Rockville, MD, USA). Balb/3T3 cells stably expressing red fluorescent protein (RFP) were established by transfection with RFP (EF1a)-puro lentiviral particles (AMSBIO, Cambridge, MA, USA) [11;- DLL3, green fluorescent protein (GFP), and luciferase-expressing SBC5 and 3T3 cell lines were established by transfecting RediFect Red-FLuc lentiviral particles (PerkinElmer, Waltham, MA, USA) and DLL3-GFP lentiviral particles (OriGene, Rockville, MD, USA);DLL3, green fluorescent protein (GFP), and luciferase-expressing SBC5 and 3T3 cell lines were established by transfecting RediFect Red-FLuc lentiviral particles (PerkinElmer, Waltham, MA, USA) and DLL3-GFP lentiviral particles (OriGene, Rockville, MD, USA);
DLL3 regulates the migration and invasion of small cell lung cancer by modulating Snail
Furuta, Kikuchi, Shoji et al
Cancer Sci. (2019) 110 (5), 1599-1608 DOI: 10.1111/cas.13997
Furuta, Kikuchi, Shoji et al
Cancer Sci. (2019) 110 (5), 1599-1608 DOI: 10.1111/cas.13997
AI Summary: OriGene products, specifically the DLL3 shRNA (TL313874) and DLL3 cDNA (RC208659), were used in this study to knock down and overexpress DLL3 in small cell lung cancer cell lines, enabling the authors to demonstrate DLL3’s role in promoting cell migration, invasion, and tumor growth.
Showing 1-2 of 2 papers.
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