DLL3 (NM_016941) Human Over-expression Lysate
$206.00
In Stock*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC200005 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_016941, NP_058637 |
| Synonyms | SCDO1 |
| Predicted MW | 64.6 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_058637 |
| Locus ID | 10683 |
| Cytogenetics | 19q13.2 |
| Protein Families | WB |
| Protein Pathways | Notch signaling pathway |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "DLL3" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LY413792 | Transient overexpression lysate of delta-like 3 (Drosophila) (DLL3), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
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Citations
Near infrared photoimmunotherapy targeting DLL3 for small cell lung cancer
Isobe, Sato, Nishinaga et al
EBioMedicine (2020) 52, 102632 DOI: 10.1016/j.ebiom.2020.102632
Isobe, Sato, Nishinaga et al
EBioMedicine (2020) 52, 102632 DOI: 10.1016/j.ebiom.2020.102632
Cited by 73
AI Summary: OriGene's DLL3-GFP lentiviral particles were used to generate DLL3-expressing cell lines, which were essential for evaluating the efficacy of DLL3-targeted near infrared photoimmunotherapy in this study.
Snippet: ein (GFP), and luciferase-expressing SBC5 and 3T3 cell lines were established by transfecting RediFect Red-FLuc lentiviral particles (PerkinElmer, Waltham, MA, USA) and DLL3-GFP lentiviral particles (OriGene, Rockville, MD, USA). Balb/3T3 cells stably expressing red fluorescent protein (RFP) were established by transfection with RFP (EF1a)-puro lentiviral particles (AMSBIO, Cambridge, MA, USA) [11;- DLL3, green fluorescent protein (GFP), and luciferase-expressing SBC5 and 3T3 cell lines were established by transfecting RediFect Red-FLuc lentiviral particles (PerkinElmer, Waltham, MA, USA) and DLL3-GFP lentiviral particles (OriGene, Rockville, MD, USA);DLL3, green fluorescent protein (GFP), and luciferase-expressing SBC5 and 3T3 cell lines were established by transfecting RediFect Red-FLuc lentiviral particles (PerkinElmer, Waltham, MA, USA) and DLL3-GFP lentiviral particles (OriGene, Rockville, MD, USA);
DLL3 regulates the migration and invasion of small cell lung cancer by modulating Snail
Furuta, Kikuchi, Shoji et al
Cancer Sci. (2019) 110 (5), 1599-1608 DOI: 10.1111/cas.13997
Furuta, Kikuchi, Shoji et al
Cancer Sci. (2019) 110 (5), 1599-1608 DOI: 10.1111/cas.13997
AI Summary: OriGene products, specifically the DLL3 shRNA (TL313874) and DLL3 cDNA (RC208659), were used in this study to knock down and overexpress DLL3 in small cell lung cancer cell lines, enabling the authors to demonstrate DLL3’s role in promoting cell migration, invasion, and tumor growth.
Showing 1-2 of 2 papers.
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