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SOX18 (NM_018419) Human Over-expression Lysate

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SKU
LY413065
Transient overexpression lysate of SRY (sex determining region Y)-box 18 (SOX18) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210323
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_018419, NP_060889
Synonyms HLTRS; HLTS
Predicted MW 40.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_060889
Locus ID 54345
Cytogenetics 20q13.33
Protein Families WB
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Other "SOX18" proteins (3)
SKU Description Size Price
PH310323 SOX18 MS Standard C13 and N15-labeled recombinant protein (NP_060889) 10 ug
$3,360.00
LC413065 Lyophilized SOX18 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP310323 Recombinant protein of human SRY (sex determining region Y)-box 18 (SOX18), 20 µg 20 ug
$867.00

file-alt Citations

Functional domain analysis of SOX18 transcription factor using a single-chain variable fragment-based approach
Fontaine, Goodall, Prokop et al
MAbs (2018)
AI Summary: OriGene’s human SOX18 and SOX2 HEK lysates were used as protein sources in immunoblot assays to assess the binding specificity of the engineered F5 monoclonal antibody in this study.
Snippet: The binding of F5 MAb to human SOX18 and human SOX2 expressed in HEK lysates (Origene) was also determined by immunoblot with HEK lysate alone as a control
Showing 1-1 of 1 papers.
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