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S100A4 (NM_019554) Human Over-expression Lysate

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SKU
LY412695
Transient overexpression lysate of S100 calcium binding protein A4 (S100A4), transcript variant 2 (as WB positive control)
  • LY412695 is the same product as LY429599.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC201616
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_019554, NP_062427
Synonyms 18A2; 42A; CAPL; FSP1; MTS1; P9KA; PEL98
Predicted MW 11.7 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_062427
Locus ID 6275
Cytogenetics 1q21.3
Protein Families WB
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Other "S100A4" proteins (7)
SKU Description Size Price
PH301616 S100A4 MS Standard C13 and N15-labeled recombinant protein (NP_062427) 10 ug
$3,360.00
PH303035 S100A4 MS Standard C13 and N15-labeled recombinant protein (NP_002952) 10 ug
$3,360.00
LC412695 Lyophilized S100A4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC418991 Lyophilized S100A4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY418991 Transient overexpression lysate of S100 calcium binding protein A4 (S100A4), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP301616 Recombinant protein of human S100 calcium binding protein A4 (S100A4), transcript variant 2, 20 µg 20 ug
$737.00
TP303035 Recombinant protein of human S100 calcium binding protein A4 (S100A4), transcript variant 1, 20 µg 20 ug
$737.00

file-alt Citations

Quantitative Proteomics Reveal an Altered Pattern of Protein Expression in Brain Tissue from Mice Lacking GPR37 and GPR37L1
Nguyen, Dammer, Owino et al
J. Proteome Res. (2020) 19 (2), 744-755 DOI: 10.1021/acs.jproteome.9b00622
Cited by 14
AI Summary: OriGene products—specifically human S100A5-myc-DDK, human S100A4-myc-DDK, and human S100A6-myc-DDK—were used in this study to generate cell lysates for validating S100A5 antibody specificity in HEK293T cells.
Snippet: human S100A5-myc-DDK (Origene), human S100A4-myc-DDK (Origene), and human S100A6- myc-DDK (Origene). Cell lysate preparation S100A5 antibody validation Lysates from HEK293T cells that were transfected with either S100A4-mycDDK (Origene);- human S100A5-myc-DDK (Origene), human S100A4-myc-DDK (Origene), and human S100A6- myc-DDK (Origene). Cell lysate preparation S100A5 antibody validation Lysates from HEK293T cells that were transfected with either S100A4-mycDDK (Origene)
Overexpression of S100A4 in human cancer cell lines resistant to methotrexate
Mencía, Selga, Rico et al
BMC Cancer (2010) 10, 250 DOI: 10.1186/1471-2407-10-250
Cited by 36
AI Summary: In this study, OriGene’s S100A4 expression vector (RC209307) and S100A4 siRNA (SR304274) were used to modulate S100A4 levels in cancer cell lines, enabling the authors to demonstrate that S100A4 overexpression contributes to methotrexate resistance while its knockdown sensitizes cells to the drug.
FigureFigureFigure
Showing 1-2 of 2 papers.
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