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PLSCR3 (NM_020360) Human Over-expression Lysate

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SKU
LY412530
Transient overexpression lysate of phospholipid scramblase 3 (PLSCR3) (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210587
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_020360, NP_065093
Predicted MW 31.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_065093
Locus ID 57048
Cytogenetics 17p13.1
Protein Families WB
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Other "PLSCR3" proteins (3)
SKU Description Size Price
PH310587 PLSCR3 MS Standard C13 and N15-labeled recombinant protein (NP_065093) 10 ug
$3,360.00
LC412530 Lyophilized PLSCR3 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP310587 Recombinant protein of human phospholipid scramblase 3 (PLSCR3), 20 µg 20 ug
$867.00

file-alt Citations

Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3
Merrick, Dhungana, Williams et al
Mol. Cell Proteomics (2011) 10 (10), M110.006007 DOI: 10.1074/mcp.M110.006007
Cited by 12
AI Summary: OriGene contributed to this study by providing the pCMV6 expression plasmid for murine Plscr3, which was used for site-directed mutagenesis experiments to investigate the role of palmitoylation in mitochondrial targeting.
Snippet: Site-directed Mutagenesis of Plscr3. A pCMV6 expression plasmid for murine Plscr3 was obtained from Origene (Rockville, MD). Cys→Ala mutations were introduced using the QuickChange Site-Directed Mutagenesis kit (Stratagene, La Jolla, CA).
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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