PLSCR3 (NM_020360) Human Tagged ORF Clone
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | PLSCR3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_020360 |
| ORF Size | 885 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_020360.4 |
| RefSeq Size | 2089 bp |
| RefSeq ORF | 888 bp |
| Locus ID | 57048 |
| UniProt ID | Q9NRY6 |
| Cytogenetics | 17p13.1 |
| Domains | Scramblase |
| MW | 31.6 kDa |
| Summary | May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. Seems to play a role in apoptosis, through translocation of cardiolipin from the inner to the outer mitochondrial membrane which promotes BID recruitment and enhances tBid-induced mitochondrial damages.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_020360" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210587L3 | Lenti ORF clone of Human phospholipid scramblase 3 (PLSCR3), transcript variant 1, Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC210587L4 | Lenti ORF clone of Human phospholipid scramblase 3 (PLSCR3), transcript variant 1, mGFP tagged | 10 ug |
$600.00
|
|
| RG210587 | PLSCR3 (tGFP-tagged) - Human phospholipid scramblase 3 (PLSCR3), transcript variant 1 | 10 ug |
$489.00
$500.00
|
|
| SC113138 | PLSCR3 (untagged)-Human phospholipid scramblase 3 (PLSCR3), transcript variant 1 | 10 ug |
$300.00
|
|
Citations
TGFB signaling induces mitophagy via PLSCR3-mediated cardiolipin externalization in conjunction with a BNIP3L/NIX-, BNIP3-, and FUNDC1-dependent mechanism
Yan, Chen, Choksi et al
Autophagy (2025)
Yan, Chen, Choksi et al
Autophagy (2025)
Cited by 16
AI Summary: OriGene’s pCMV6-PLSCR3 plasmid (RC210587) was used as the source for PLSCR3 constructs in this study to investigate the role of PLSCR3 in TGFB1-induced mitophagy.
Snippet: ubcloned from pCMV6-PLSCR3 (OriGene, RC210587) into the pMXs rtv-016 backbone released from pMXs-3×HA-EGFP-OMP25 using XhoI and NotI restriction enzymes. The PLSCR3 F258V mutant was generated using Q5 site-directed mutagenesis kit (New England Biolabs, E0554S) with pMXs-PLSCR3 WT as the templat
Ferroptosis induces nucleolar stress as revealed by live-cell imaging using thioflavin T
Hirata, Takemori, Furuta et al
Curr Res Pharmacol Drug Discov (2024) 7, 100196 DOI: 10.1016/j.crphar.2024.100196
Hirata, Takemori, Furuta et al
Curr Res Pharmacol Drug Discov (2024) 7, 100196 DOI: 10.1016/j.crphar.2024.100196
Cited by 3
Snippet: mont, IL, USA, RRID:AB_2155162), anti-glutathione peroxidase 4 (GPX4) antibody (1:1000;abcam, Cambridge, UK, RRID:AB_10973901), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (1:5000;OriGene Technologies, Rockville, MD, USA, cat.# ACR001P) as the gel loading control. The immunolabeled membranes were then probed with a secondary antibody for 90 min at room temperature (RT) and tar
Genome-Wide CRISPR Screen Identifies Phospholipid Scramblase 3 as the Biological Target of Mitoprotective Drug SS-31
Silvaroli, Bisunke, Kim et al
J Am Soc Nephrol (2024)
Silvaroli, Bisunke, Kim et al
J Am Soc Nephrol (2024)
Proteomic profiling of S-acylated macrophage proteins identifies a role for palmitoylation in mitochondrial targeting of phospholipid scramblase 3
Merrick, Dhungana, Williams et al
Mol. Cell Proteomics (2011) 10 (10), M110.006007 DOI: 10.1074/mcp.M110.006007
Merrick, Dhungana, Williams et al
Mol. Cell Proteomics (2011) 10 (10), M110.006007 DOI: 10.1074/mcp.M110.006007
Cited by 12
AI Summary: OriGene contributed to this study by providing the pCMV6 expression plasmid for murine Plscr3, which was used for site-directed mutagenesis experiments to investigate the role of palmitoylation in mitochondrial targeting.
Showing 1-4 of 4 papers.
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