PLSCR3 (NM_020360) Human Untagged Clone

SKU SC113138

PLSCR3 (untagged)-Human phospholipid scramblase 3 (PLSCR3), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PLSCR3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_020360, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCAGGCTACTTGCCCCCCAAAGGCTACGCCCCTTCGCCCCCACCTCCCTACCCTGTCACCCCTGGGT; ACCCGGAGCCGGCGCTACATCCTGGGCCCGGGCAGGCGCCAGTGCCCGCCCAGGTACCTGCCCCAGCTCC; CGGCTTCGCCCTCTTCCCCTCGCCTGGCCCCGTGGCCTTGGGGTCTGCTGCCCCCTTCTTGCCACTGCCA; GGGGTGCCTTCTGGCCTCGAATTCCTGGTGCAGATTGATCAGATTTTGATTCACCAGAAGGCTGAGCGAG; TGGAAACGTTCCTAGGCTGGGAGACCTGTAATCGGTATGAACTGCGCTCTGGGGCCGGGCAGCCCCTGGG; TCAGGCGGCCGAGGAGAGCAACTGCTGCGCCCGTCTGTGCTGTGGCGCCCGCCGGCCGCTGCGTGTCCGC; CTGGCCGACCCCGGGGACCGTGAGGTGCTGCGTTTGCTCCGCCCGCTGCACTGTGGCTGCAGCTGCTGCC; CCTGTGGCCTCCAGGAGATGGAAGTACAGGCTCCACCAGGCACCACCATTGGCCACGTGCTACAGACCTG; GCATCCCTTCCTCCCCAAGTTCTCCATCCAGGATGCCGATCGCCAGACAGTCTTGCGAGTGGTGGGGCCC; TGCTGGACCTGTGGCTGTGGCACAGACACCAACTTTGAGGTGAAGACTCGGGATGAATCCCGCAGTGTGG; GCCGCATCAGCAAGCAGTGGGGGGGCCTGGTCCGAGAAGCCCTCACAGATGCAGATGACTTTGGCCTACA; GTTCCCGCTGGACCTGGATGTGAGGGTGAAGGCTGTGCTGCTGGGAGCCACATTCCTCATTGACTACATG; TTCTTTGAGAAGCGAGGAGGCGCTGGGCCCTCTGCCGTCACCAGTTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020360 unedited; GGGGTCGCAATTGTATACGACTCACTATAGGGCGGCCGCGAATCGGCACGAGGGTGCTAG; GCACCCGGGCTCTTCTGGGGGCTCCAGAACTAAGCCACCCAGACACCATCATCTCGAAAA; CCCCAGCCCTTCTCCCATGGCAGGTAAGTGCGCGCAAGTCTGGGGGTCTCCGTTATCCCC; CCCACGGACTTGGGAGCCGGGTTTGAACACATCCTCCCGGAATGTGCTCGGACTGGCTGG; GTGGGGCCCCTCCCTTCCTTCCGGGGAACCTCCACCCTGCTCCCCTGCCCCATCTTTCTC; TGCCTCTTGCCACCACGTTTCCCCCGAAGCCTCGCGGCCCATGGGCTGAGGAATAAGGGG; ATGAGGCCTTGAGTCCCTTTCTAATCTATCCTGCTCCCACCGCTTTACAGGCTACTTGCC; CCCCAAAGGCTACGCCCCTTCGCCCCCACCTCCCTACCCTGTCACCCCTGGGTACCCGGA; GCCGGCGCTACATCCTGGGCCCGGGCAGGCGCCAGTGCCCGCCCAGGTACCTGCCCCAGC; TCCCGGCTTCGCCCTCTTCCCCTCGCCTGGCCCCGTGGCCTTGGGGTCTGCTGCCCCCTT; CTTGCCACTGCCAGGGGTGCCTTCTGGCCTCGAATTCCTGGTGCAGATTGATCAGATTTT; GATTCACCAGAAAGCTGAGCGAGTGGAAAGTAAGTAAAAGGAGTGGGAGGTTGGACCTCA; TGATGGTGGCCCGGATGGTGGGCTCCTGNGCGCGCGAAGCTCTACGAGTCTCCCCGTGTC; ACCNTCGGCAGCCGTTCTAAGCTGGGAGACCTGTTATCGGTATGAACTGCGCTCTGGGGC; CGGGCAGCCCCTGGGTCAAGCGGCCCGAAGAGACAACTGCTGCGCCCGTCTGTGCTGTGG; CGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020360 unedited; GGCTTCTTGNACCCGGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTTTGTCCAAA; AGTTACAAAGTTTATATGAATGTAACATATAAAGATGCAGCCTTTTTCCTAGGGCAAGGG; AAGGAAGGGCAGTTACATAAGGCACAGGAATCGCAGCAAGCACCAGCTGAAGGCAGGGCC; CTAGTGGGAGAGGAAGTTGGCACTGGGGTCCCCCTGCAGCTGTTGATGGGGAGGTAGAGA; CCCCCTCAAAGTGCCATGGTCTGAGGCTGAACTAATTGGCCAAGCAGATGCTAGGCAGCG; GCGGGGGGGCGGTGGCTATATCCACCGTCTTTGGTGCAGCTTGCCCTTCCACACCCCAGC; CCTCTTGTAAAACCCCAGAGTCCTGGGTTCCAGAGGCCTCCTTTGGAGCAGAGGCCNCTG; GAAGGGGGTGGGAGAGAGTGCATGGGGAAAGTGTGAAAGCTGATATGCCTGTGTGCCGAG; GGACTGCTGGGACAGTGGTGGGAGGTANCANGAGAGCGGCTCTCATACATACCCCTCCTT; GGGAACCACAGGGGCAGGCGGCCANGGAGTANGGTNGGGGATGGGGGCCCCCCTTCTGTC; CCCTGCAGTGTACATGGAGGAAAGGGGCTGCCCANAGGAGGGGCCAGGGCANGTGACCAT; CTGGAGTTCTGGGTCNAGTGATGGTCTCCTCACACAATGGTGGNNCTCTACTGGTGACGG; CAGAGGGCCCANCGCCTNCTCGCTTCTCAAAGACATGTAGTCATGAGGAATGTGGGTCCC; ACAGACAGGCTTTACCTTACATTCAGGTCCACGGGACTGTAGGCAAAGCATCTGCTTTGG; TGAGGCTTTTTGGGACAAGCCCCCCACTGTTTGTTGTGCCGCCCAATGGGGGATATCCCC; GGCCTACCTAAAAAGGGGGGGCTGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_020360
• Insert Size: 2260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020360.2, NP_065093.2
• RefSeq Size: 1746 bp
• RefSeq ORF: 888 bp
• Locus ID: 57048
• UniProt ID: Q9NRY6
• Cytogenetics: 17p13.1
• Domains: Scramblase
• Summary: May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. Seems to play a role in apoptosis, through translocation of cardiolipin from the inner to the outer mitochondrial membrane which promotes BID recruitment and enhances tBid-induced mitochondrial damages.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.