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ERGIC1 (NM_020462) Human Over-expression Lysate

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SKU
LY412466
Transient overexpression lysate of endoplasmic reticulum-golgi intermediate compartment (ERGIC) 1 (ERGIC1), transcript variant 2 (as WB positive control)
  • LY412466 is the same product as LY429623.
Size:
100 ug 20 ug
Formulation:
Liquid Lyophilized
  $436.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC222998
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_020462, NP_065195
Synonyms endoplasmic reticulum-golgi intermediate compartment (ERGIC) 1; endoplasmic reticulum-golgi intermediate compartment 32 kDa protein; ERGIC-32; ERGIC32; ERGIC32, ERGIC-32, KIAA1181, MGC14345; FLJ39864; KIAA1181; MGC14345
Predicted MW 18.2 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_065195
Locus ID 57222
Cytogenetics 5q35.1
Protein Families WB
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Other "ERGIC1" proteins (5)
SKU Description Size Price
PH303612 ERGIC1 MS Standard C13 and N15-labeled recombinant protein (NP_001026881) 10 ug
$3,360.00
LC412466 Lyophilized ERGIC1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC422162 Lyophilized ERGIC1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY422162 Transient overexpression lysate of endoplasmic reticulum-golgi intermediate compartment (ERGIC) 1 (ERGIC1) (as WB positive control) 100 ug
$436.00
TP303612 Recombinant protein of human endoplasmic reticulum-golgi intermediate compartment (ERGIC) 1 (ERGIC1), 20 µg 20 ug
$867.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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