OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

HKDC1 (NM_025130) Human Over-expression Lysate

Be the first to review this product
SKU
LY410883
Transient overexpression lysate of hexokinase domain containing 1 (HKDC1) (as WB positive control)
  $665.00
In Stock*
Bulk/Customize

Frequently Bought Together (2)

TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "HKDC1 or NM_025130"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221178
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_025130, NP_079406
Predicted MW 102.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_079406
Locus ID 80201
Cytogenetics 10q22.1
Protein Families WB
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "HKDC1" proteins (3)
SKU Description Size Price
PH321178 HKDC1 MS Standard C13 and N15-labeled recombinant protein (NP_079406) 10 ug
$3,360.00
LC410883 Lyophilized HKDC1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP321178 Recombinant protein of human hexokinase domain containing 1 (HKDC1), 20 µg 20 ug
$737.00

file-alt Citations

Hepatic hexokinase domain containing 1 (HKDC1) improves whole body glucose tolerance and insulin sensitivity in pregnant mice
Wasim Khan, Priyadarshini, Cordoba-Chacon et al
Biochim Biophys Acta Mol Basis Dis (2018)
AI Summary: OriGene's RC221178 plasmid, containing the 2750 bp open reading frame of HKDC1, was used as the source of the human HKDC1 gene for constructing the adenoviral vector to overexpress HKDC1 in mouse liver in this study.
Snippet: described [21]. In brief, fragment containing the 2750 bp ORF of HKDC1 (RC221178 plasmid, Origene, Rockville, MD, USA), was cloned into the pShuttle-CMV adenoviral vector (Agilent, Santa Clara, CA, USA). The resulting
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products