HKDC1 (NM_025130) Human Tagged ORF Clone
$1,259.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | HKDC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_025130 |
| ORF Size | 2751 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_025130.3, NP_079406.3 |
| RefSeq Size | 3719 bp |
| RefSeq ORF | 2754 bp |
| Locus ID | 80201 |
| UniProt ID | Q2TB90 |
| Cytogenetics | 10q22.1 |
| Domains | hexokinase |
| Protein Families | Druggable Genome |
| MW | 102.5 kDa |
| Summary | This gene encodes a member of the hexokinase protein family. The encoded protein is involved in glucose metabolism, and reduced expression may be associated with gestational diabetes mellitus. High expression of this gene may also be associated with poor prognosis in hepatocarcinoma. provided by RefSeq, Sep 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_025130" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC221178L1 | Lenti ORF clone of Human hexokinase domain containing 1 (HKDC1), Myc-DDK-tagged | 10 ug |
$1,559.00
|
|
| RC221178L2 | Lenti ORF clone of Human hexokinase domain containing 1 (HKDC1), mGFP tagged | 10 ug |
$1,559.00
|
|
| RC221178L3 | Lenti ORF clone of Human hexokinase domain containing 1 (HKDC1), Myc-DDK-tagged | 10 ug |
$1,559.00
|
|
| RC221178L4 | Lenti ORF clone of Human hexokinase domain containing 1 (HKDC1), mGFP tagged | 10 ug |
$1,559.00
|
|
| RG221178 | HKDC1 (tGFP-tagged) - Human hexokinase domain containing 1 (HKDC1) | 10 ug |
$1,459.00
|
|
| SC314919 | HKDC1 (untagged)-Human hexokinase domain containing 1 (HKDC1) | 10 ug |
$1,344.00
|
|
Citations
Epigenetic reprogramming of hexokinase domain containing 1 (HKDC1) promotes proliferation in colorectal cancer
Chen, Lai, Lin et al
Am J Cancer Res (2026) 16 (3), 1013-1026 DOI: 10.62347/OSSN5233
Chen, Lai, Lin et al
Am J Cancer Res (2026) 16 (3), 1013-1026 DOI: 10.62347/OSSN5233
AI Summary: OriGene’s pCMV6-HKDC1 (RC221178) and pCMV6-Entry vectors were used for HKDC1 overexpression and control transfection experiments, enabling the study of HKDC1’s role in promoting colorectal cancer cell proliferation.
Snippet: ssay AID: 504763) in PubChem (https://pubchem.ncbi.nlm.nih.gov/compound/68262#section=Biological-Test-Results). HKDC1 TRANSFECTION pCMV6-HKDC1 (RC221178) and pCMV6-Entry vectors were purchased from OriGene Technologies (Rockville, MD, USA). Transfection was performed in Opti-MEM (Gibco) using a mix containing Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) and 10 μg plasmid, for 24 h at 3
Hepatic hexokinase domain containing 1 (HKDC1) improves whole body glucose tolerance and insulin sensitivity in pregnant mice
Wasim Khan, Priyadarshini, Cordoba-Chacon et al
Biochim Biophys Acta Mol Basis Dis (2018)
Wasim Khan, Priyadarshini, Cordoba-Chacon et al
Biochim Biophys Acta Mol Basis Dis (2018)
Coordinated regulatory variation associated with gestational hyperglycaemia regulates expression of the novel hexokinase HKDC1
Guo, Ludvik, Arlotto et al
Nat Commun (2015) 6, 6069 DOI: 10.1038/ncomms7069
Guo, Ludvik, Arlotto et al
Nat Commun (2015) 6, 6069 DOI: 10.1038/ncomms7069
Cited by 110
AI Summary: OriGene’s HKDC1 cDNA clone (plasmid RC221178) was used to provide the HKDC1 open reading frame for generating adenoviral vectors to study the gene’s function in regulating glucose metabolism during pregnancy.
Snippet: A 2,789 bp BamHI/NotI fragment from plasmid RC221178 (OriGene), containing the 2,750 bp HKDC1 open reading frame, was cloned into the BamHI/NotI sites of the adenoviral shuttle vector pShuttle-CMV (Agilent). HKDC1 adenoviral shuttles were linearized with PmeI and transformed into BJ5183-AD1 cells (Agilent)
Showing 1-3 of 3 papers.
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