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TMEM79 (NM_032323) Human Over-expression Lysate

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SKU
LY410199
Transient overexpression lysate of transmembrane protein 79 (TMEM79), transcript variant 1 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202998
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_032323, NP_115699
Synonyms MATT
Predicted MW 43.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_115699
Locus ID 84283
Cytogenetics 1q22
Protein Families WB
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Other "TMEM79" proteins (3)
SKU Description Size Price
PH302998 TMEM79 MS Standard C13 and N15-labeled recombinant protein (NP_115699) 10 ug
$3,360.00
LC410199 Lyophilized TMEM79 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP302998 Recombinant protein of human transmembrane protein 79 (TMEM79), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Tmem79/Matt is the matted mouse gene and is a predisposing gene for atopic dermatitis in human subjects
Saunders, Goh, Brown et al
J. Allergy Clin. Immunol. (2013) 132 (5), 1121-9 DOI: 10.1016/j.jaci.2013.08.046
Cited by 161
AI Summary: OriGene’s Human cDNA Tissue Array was used to quantify TMEM79/MATT gene expression across 48 different human tissues, aiding in the analysis of this gene's role in atopic dermatitis.
Snippet: Germany). Quantitative PCR of human cDNA tissue array. Expression of TMEM79/MATT cDNA was quantified across a multiple-tissue panel containing cDNA from 48 different human tissues (Origene Technologies, Rockville, Md).
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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