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TMEM79 (NM_032323) Human Over-expression Lysate

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SKU
LC410199
Lyophilized TMEM79 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC202998
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_032323, NP_115699
Synonyms MATT
Predicted MW 43.5 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_115699
Locus ID 84283
Cytogenetics 1q22
Protein Families WB
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Other "TMEM79" proteins (3)
SKU Description Size Price
PH302998 TMEM79 MS Standard C13 and N15-labeled recombinant protein (NP_115699) 10 ug
$3,360.00
LY410199 Transient overexpression lysate of transmembrane protein 79 (TMEM79), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP302998 Recombinant protein of human transmembrane protein 79 (TMEM79), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

Tmem79/Matt is the matted mouse gene and is a predisposing gene for atopic dermatitis in human subjects
Saunders, Goh, Brown et al
J. Allergy Clin. Immunol. (2013) 132 (5), 1121-9 DOI: 10.1016/j.jaci.2013.08.046
Cited by 161
AI Summary: OriGene’s Human cDNA Tissue Array was used to quantify TMEM79/MATT gene expression across 48 different human tissues, aiding in the analysis of this gene's role in atopic dermatitis.
Snippet: Germany). Quantitative PCR of human cDNA tissue array. Expression of TMEM79/MATT cDNA was quantified across a multiple-tissue panel containing cDNA from 48 different human tissues (Origene Technologies, Rockville, Md).
Showing 1-1 of 1 papers.
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