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CABS1 (NM_033122) Human Over-expression Lysate

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SKU
LY409727
Transient overexpression lysate of chromosome 4 open reading frame 35 (C4orf35) (as WB positive control)
  $436.00
4 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208496
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_033122, NP_149113
Synonyms C4orf35; CLPH; NYD-SP26
Predicted MW 42.9 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_149113
Locus ID 85438
Cytogenetics 4q13.3
Protein Families WB
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Other "CABS1" proteins (3)
SKU Description Size Price
PH308496 C4orf35 MS Standard C13 and N15-labeled recombinant protein (NP_149113) 10 ug
$3,360.00
LC409727 Lyophilized CABS1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP308496 Recombinant protein of human chromosome 4 open reading frame 35 (C4orf35), 20 µg 20 ug
$867.00

file-alt Citations

A method to study protein biomarkers in saliva using an automated capillary nano-immunoassay platform (Wes )
Reyes-Serratos, Marcet-Palacios, Rosenfield et al
J Immunol Methods (2020) 479, 112749 DOI: 10.1016/j.jim.2020.112749
Cited by 8
AI Summary: In this study, OriGene's anti-hCABS1 antibody (Cat# TA322395) was used to detect and validate the novel biomarker hCABS1 in saliva samples, enabling comparison of Western blot and automated capillary nano-immunoassay (Wes™) platforms.
FigureFigure
Calcium-binding protein, spermatid-specific 1 is expressed in human salivary glands and contains an anti-inflammatory motif
St Laurent, St Laurent, Mathison et al
Am J Physiol Regul Integr Comp Physiol (2015) 308 (7), R569-75 DOI: 10.1152/ajpregu.00153.2014
Cited by 20
AI Summary: OriGene products, specifically the CABS1 cDNA clone (Cat# RC217845) and anti-CABS1 antibody (Cat# TA323113), were used in this study to detect and analyze CABS1 expression in human tissues via PCR and Western blot, supporting the discovery of its presence in salivary glands and its potential anti-inflammatory function.
Showing 1-2 of 2 papers.
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