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CABS1 (NM_033122) Human Over-expression Lysate

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SKU
LC409727
Lyophilized CABS1 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC208496
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_033122, NP_149113
Synonyms C4orf35; CLPH; NYD-SP26
Predicted MW 42.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_149113
Locus ID 85438
Cytogenetics 4q13.3
Protein Families WB
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Other "CABS1" proteins (3)
SKU Description Size Price
PH308496 C4orf35 MS Standard C13 and N15-labeled recombinant protein (NP_149113) 10 ug
$3,360.00
LY409727 Transient overexpression lysate of chromosome 4 open reading frame 35 (C4orf35) (as WB positive control) 100 ug
$436.00
TP308496 Recombinant protein of human chromosome 4 open reading frame 35 (C4orf35), 20 µg 20 ug
$867.00

file-alt Citations

A method to study protein biomarkers in saliva using an automated capillary nano-immunoassay platform (Wes )
Reyes-Serratos, Marcet-Palacios, Rosenfield et al
J Immunol Methods (2020) 479, 112749 DOI: 10.1016/j.jim.2020.112749
Cited by 8
AI Summary: In this study, OriGene's anti-hCABS1 antibody (Cat# TA322395) was used to detect and validate the novel biomarker hCABS1 in saliva samples, enabling comparison of Western blot and automated capillary nano-immunoassay (Wes™) platforms.
FigureFigure
Calcium-binding protein, spermatid-specific 1 is expressed in human salivary glands and contains an anti-inflammatory motif
St Laurent, St Laurent, Mathison et al
Am J Physiol Regul Integr Comp Physiol (2015) 308 (7), R569-75 DOI: 10.1152/ajpregu.00153.2014
Cited by 20
AI Summary: OriGene products, specifically the CABS1 cDNA clone (Cat# RC217845) and anti-CABS1 antibody (Cat# TA323113), were used in this study to detect and analyze CABS1 expression in human tissues via PCR and Western blot, supporting the discovery of its presence in salivary glands and its potential anti-inflammatory function.
Showing 1-2 of 2 papers.
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