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BORIS (CTCFL) (NM_080618) Human Over-expression Lysate

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SKU
LY409159
Transient overexpression lysate of CCCTC-binding factor (zinc finger protein)-like (CTCFL) (as WB positive control)
  $665.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC216042
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_080618, NP_542185
Synonyms BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1
Predicted MW 75.6 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_542185
Locus ID 140690
Cytogenetics 20q13.31
Protein Families WB
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Other "BORIS" proteins (2)
SKU Description Size Price
LC409159 Lyophilized CTCFL HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
TP762263 Purified recombinant protein of Human CCCTC-binding factor (zinc finger protein)-like (CTCFL), Glu282-Thr435, with N-terminal His tag, expressed in E.coli, 50ug 50 ug
$260.00

file-alt Citations

Investigation of fusion gene expression in HCT116 cells
Zhang, Ren, Fang et al
Oncol Lett (2017) 14 (6), 6962-6968 DOI: 10.3892/ol.2017.7055
Cited by 11
AI Summary: OriGene products, specifically the BORIS (RC216307) and CTCF (RC216307) expression plasmids, were used in this study to modulate and investigate the regulation of fusion gene expression in HCT116 colon cancer cells.
BORIS binding to the promoters of cancer testis antigens, MAGEA2, MAGEA3, and MAGEA4, is associated with their transcriptional activation in lung cancer
Bhan, Negi, Shao et al
Clin. Cancer Res. (2011) 17 (13), 4267-76 DOI: 10.1158/1078-0432.CCR-11-0653
Cited by 8
AI Summary: OriGene’s BORIS shRNA plasmids were used to knock down BORIS expression in H1299 cells, enabling the study of BORIS’s role in regulating MAGEA2, MAGEA3, and MAGEA4 gene expression in lung cancer.
Snippet: streptomycin and incubated at 37°C and 5% CO 2 . Small hairpin RNA (shRNA) plasmids for BORIS knockdown were purchased from Origene. ... To evaluate the effect of BORIS on the expression of the selected targets, we knocked down the expression of BORIS in H1299 cells
Showing 1-2 of 2 papers.
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