BORIS (CTCFL) (NM_080618) Human Tagged ORF Clone
- TrueORF®
SKU
RC216042
CTCFL (Myc-DDK-tagged)-Human CCCTC-binding factor (zinc finger protein)-like (CTCFL)
$925.00
2 Weeks*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | BORIS |
| Synonyms | BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_080618 |
| ORF Size | 1989 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_080618.1 |
| RefSeq Size | 3493 bp |
| RefSeq ORF | 1992 bp |
| Locus ID | 140690 |
| UniProt ID | Q8NI51 |
| Cytogenetics | 20q13.31 |
| Protein Families | Transcription Factors |
| MW | 75.6 kDa |
| Summary | CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jun 2012 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_080618" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC216042L1 | Lenti ORF clone of Human CCCTC-binding factor (zinc finger protein)-like (CTCFL), Myc-DDK-tagged | 10 ug |
$1,225.00
|
|
| RC216042L2 | Lenti ORF clone of Human CCCTC-binding factor (zinc finger protein)-like (CTCFL), mGFP tagged | 10 ug |
$1,225.00
|
|
| RC216042L3 | Lenti ORF clone of Human CCCTC-binding factor (zinc finger protein)-like (CTCFL), Myc-DDK-tagged | 10 ug |
$1,225.00
|
|
| RC216042L4 | Lenti ORF clone of Human CCCTC-binding factor (zinc finger protein)-like (CTCFL), mGFP tagged | 10 ug |
$1,225.00
|
|
| RG216042 | CTCFL (tGFP-tagged) - Human CCCTC-binding factor (zinc finger protein)-like (CTCFL) | 10 ug |
$1,125.00
|
|
| SC311151 | CTCFL (untagged)-Human CCCTC-binding factor (zinc finger protein)-like (CTCFL) | 10 ug |
$927.00
|
|
Citations
Boris knockout eliminates AOM/DSS-induced in situ colorectal cancer by suppressing DNA damage repair and inflammation
Zuo, Yao, Fang et al
Cancer Sci (2023) 114 (5), 1972-1985 DOI: 10.1111/cas.15732
Zuo, Yao, Fang et al
Cancer Sci (2023) 114 (5), 1972-1985 DOI: 10.1111/cas.15732
Cited by 11
AI Summary: In this study, OriGene products including the Boris (CTCFL) expression plasmid (RC210377) and Boris (CTCFL) shRNA (TL516952) were used to verify the function of Boris in colorectal cell lines and to assess the effects of Boris knockdown, thereby supporting the investigation of Boris's role in colorectal cancer progression and inflammation.
Snippet: … However, it is unknown whether Boris plays a role in the progression of in situ colorectal cancer. …The importance of Boris is emphasized in the progression of in situ colorectal cancer. …
Investigation of fusion gene expression in HCT116 cells
Zhang, Ren, Fang et al
Oncol Lett (2017) 14 (6), 6962-6968 DOI: 10.3892/ol.2017.7055
Zhang, Ren, Fang et al
Oncol Lett (2017) 14 (6), 6962-6968 DOI: 10.3892/ol.2017.7055
Cited by 11
AI Summary: OriGene products, specifically the BORIS (RC216307) and CTCF (RC216307) expression plasmids, were used in this study to modulate and investigate the regulation of fusion gene expression in HCT116 colon cancer cells.
BORIS/CTCFL-mediated transcriptional regulation of the hTERT telomerase gene in testicular and ovarian tumor cells
Renaud, Loukinov, Alberti et al
Nucleic Acids Res. (2011) 39 (3), 862-73 DOI: 10.1093/nar/gkq827
Renaud, Loukinov, Alberti et al
Nucleic Acids Res. (2011) 39 (3), 862-73 DOI: 10.1093/nar/gkq827
Cited by 68
AI Summary: OriGene contributed pCMV6-XL4 vectors containing BORIS cDNA and CTCF cDNA, which were used to study the effects of BORIS and CTCF expression on hTERT transcription in testicular and ovarian tumor cells.
Snippet: have reported that down-regulation of DNMTs in response to HDAC inhibition by treatment with potential cancer-prevention drug Sulforaphane ... BORIS and pCMV-CTCF vectors, BORIS cDNA and CTCF cDNA were cloned in pCMV6-XL4 by ORIGENE Technologies (Rockville
Showing 1-3 of 3 papers.
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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