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BORIS (CTCFL) (NM_080618) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG216042
CTCFL (tGFP-tagged) - Human CCCTC-binding factor (zinc finger protein)-like (CTCFL)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $1,125.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BORIS
Synonyms BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG216042 representing NM_080618
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCCACTGAGATCTCTGTCCTTTCTGAGCAATTCACCAAGATCAAAGAACTCGAGTTGATGCCGG
AAAAAGGCCTGAAGGAGGAGGAAAAAGACGGAGTGTGCAGAGAGAAAGACCATCGGAGCCCTAGTGAGTT
GGAGGCCGAGCGTACCTCTGGGGCCTTCCAGGACAGCGTCCTGGAGGAAGAAGTGGAGCTGGTGCTGGCC
CCCTCGGAGGAGAGCGAGAAGTACATCCTGACCCTGCAGACGGTGCACTTCACTTCTGAAGCTGTGGAGT
TGCAGGATATGAGCTTGCTGAGCATACAGCAGCAAGAAGGGGTGCAGGTGGTGGTGCAACAGCCTGGCCC
TGGGTTGCTGTGGCTTGAGGAAGGGCCCCGGCAGAGCCTGCAGCAGTGTGTGGCCATTAGTATCCAGCAA
GAGCTGTACTCCCCGCAAGAGATGGAGGTGTTGCAGTTCCACGCTCTAGAGGAGAATGTGATGGTGGCCA
GTGAAGACAGTAAGTTAGCGGTGAGCCTGGCTGAAACTGCTGGACTGATCAAGCTCGAGGAAGAGCAGGA
GAAGAACCAGTTATTGGCTGAAAGAACAAAGGAGCAGCTCTTTTTTGTGGAAACAATGTCAGGAGATGAA
AGAAGTGACGAAATTGTTCTCACAGTTTCAAATTCAAATGTGGAAGAACAAGAGGATCAACCTACAGCTG
GTCAAGCAGATGCTGAAAAGGCCAAATCTACAAAAAATCAAAGAAAGACAAAGGGAGCAAAAGGAACCTT
CCACTGTGATGTCTGCATGTTCACCTCTTCTAGAATGTCAAGTTTTAATCGTCATATGAAAACTCACACC
AGTGAGAAGCCTCACCTGTGTCACCTCTGCCTGAAAACCTTCCGTACGGTCACTCTGCTGCGGAACCATG
TTAACACCCACACAGGAACCAGGCCCTACAAGTGTAACGACTGCAACATGGCATTTGTCACCAGTGGAGA
ACTCGTCCGACACAGGCGCTATAAACATACTCATGAGAAACCCTTTAAATGTTCCATGTGCAAGTATGCC
AGTGTGGAGGCAAGTAAATTGAAGCGCCATGTCCGATCCCACACTGGGGAGCGCCCCTTTCAGTGTTGCC
AGTGCAGCTATGCCAGCAGAGATACCTACAAGCTGAAACGCCACATGAGAACGCACTCAGGTGAGAAGCC
TTACGAATGCCACATCTGCCACACCCGCTTCACCCAGAGCGGGACCATGAAAATACATATTCTGCAGAAA
CACGGCGAAAATGTCCCCAAATACCAGTGTCCCCATTGTGCCACCATCATTGCACGGAAAAGCGACCTAC
GTGTGCATATGCGCAACTTGCATGCTTACAGCGCTGCAGAGCTGAAATGCCGCTACTGTTCTGCTGTCTT
CCATGAACGCTATGCCCTCATTCAGCACCAGAAAACTCATAAGAATGAGAAGAGGTTCAAGTGCAAACAC
TGCAGTTATGCCTGCAAGCAGGAACGTCATATGACCGCTCACATTCGTACCCACACTGGAGAGAAACCAT
TCACCTGCCTTTCTTGCAATAAATGTTTCCGACAGAAGCAACTTCTAAACGCTCACTTCAGGAAATACCA
CGATGCAAATTTCATCCCGACTGTTTACAAATGCTCCAAGTGTGGCAAAGGCTTTTCCCGCTGGATTAAC
CTGCACAGACATTCGGAGAAGTGTGGATCAGGGGAAGCAAAGTCGGCTGCTTCAGGAAAGGGAAGAAGAA
CAAGAAAGAGGAAGCAGACCATCCTGAAGGAAGCCACAAAGGGTCAGAAGGAAGCTGCGAAGGGATGGAA
GGAAGCCGCGAACGGAGACGAAGCTGCTGCTGAGGAGGCTTCCACCACGAAGGGAGAACAGTTCCCAGGA
GAGATGTTTCCTGTCGCCTGCAGAGAAACCACAGCCAGAGTCAAAGAGGAAGTGGATGAAGGCGTGACCT
GTGAAATGCTCCTCAACACGATGGATAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080618
ORF Size 1989 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080618.1
RefSeq Size 3493 bp
RefSeq ORF 1992 bp
Locus ID 140690
UniProt ID Q8NI51
Cytogenetics 20q13.31
Protein Families Transcription Factors
Summary CCCTC-binding factor (CTCF), an 11-zinc-finger factor involved in gene regulation, utilizes different zinc fingers to bind varying DNA target sites. CTCF forms methylation-sensitive insulators that regulate X-chromosome inactivation. This gene is a paralog of CTCF and appears to be expressed primarily in the cytoplasm of spermatocytes, unlike CTCF which is expressed primarily in the nucleus of somatic cells. CTCF and the protein encoded by this gene are normally expressed in a mutually exclusive pattern that correlates with resetting of methylation marks during male germ cell differentiation. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jun 2012
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"NM_080618" in other vectors (6)
SKU Description Size Price
RC216042 CTCFL (Myc-DDK-tagged)-Human CCCTC-binding factor (zinc finger protein)-like (CTCFL) 10 ug
$925.00
RC216042L1 Lenti ORF clone of Human CCCTC-binding factor (zinc finger protein)-like (CTCFL), Myc-DDK-tagged 10 ug
$1,225.00
RC216042L2 Lenti ORF clone of Human CCCTC-binding factor (zinc finger protein)-like (CTCFL), mGFP tagged 10 ug
$1,225.00
RC216042L3 Lenti ORF clone of Human CCCTC-binding factor (zinc finger protein)-like (CTCFL), Myc-DDK-tagged 10 ug
$1,225.00
RC216042L4 Lenti ORF clone of Human CCCTC-binding factor (zinc finger protein)-like (CTCFL), mGFP tagged 10 ug
$1,225.00
SC311151 CTCFL (untagged)-Human CCCTC-binding factor (zinc finger protein)-like (CTCFL) 10 ug
$927.00

Plasmid Map for RG216042

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG216042
Circular map for RG216042

file-alt Citations

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