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TBX1 (NM_080647) Human Over-expression Lysate

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SKU
LY409117
Transient overexpression lysate of T-box 1 (TBX1), transcript variant C (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC216204
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_080647, NP_542378
Synonyms CAFS; CATCH22; CTHM; DGCR; DGS; DORV; TBX1C; TGA; VCF; VCFS
Predicted MW 52.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_542378
Locus ID 6899
Cytogenetics 22q11.21
Protein Families WB
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Other "TBX1" proteins (7)
SKU Description Size Price
PH314605 TBX1 MS Standard C13 and N15-labeled recombinant protein (NP_542377) 10 ug
$3,360.00
PH316204 TBX1 MS Standard C13 and N15-labeled recombinant protein (NP_542378) 10 ug
$3,360.00
LC409116 Lyophilized TBX1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC409117 Lyophilized TBX1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY409116 Transient overexpression lysate of T-box 1 (TBX1), transcript variant A (as WB positive control) 100 ug
$436.00
TP314605 Recombinant protein of human T-box 1 (TBX1), transcript variant A, 20 µg 20 ug
$867.00
TP316204 Purified recombinant protein of Homo sapiens T-box 1 (TBX1), transcript variant C, 20 µg 20 ug
$867.00

file-alt Citations

A loss-of-function mutation p.T52S in RIPPLY3 is a potential predisposing genetic risk factor for Chinese Han conotruncal heart defect patients without the 22q11.2 deletion/duplication
Hong, Zhang, Wang et al
J Transl Med (2018) 16 (1), 260 DOI: 10.1186/s12967-018-1633-1
Cited by 5
AI Summary: In this study, OriGene’s Human RIPPLY3 cDNA clone (RC210387) and Human TBX1 cDNA clone (RC223770) were used to investigate the functional impact of RIPPLY3 variants on TBX1 transcriptional activity and protein interactions in conotruncal heart defect patients.
FigureFigureFigure
Showing 1-1 of 1 papers.
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