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DACH1 (NM_080759) Human Over-expression Lysate

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SKU
LY409071
Transient overexpression lysate of dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1 (as WB positive control)
  $665.00
2 Weeks*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC221505
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_080759, NP_542937
Synonyms DACH
Predicted MW 73 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_542937
Locus ID 1602
Cytogenetics 13q21.33
Protein Families WB
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Documents
Resources
Other "DACH1" proteins (1)
SKU Description Size Price
LC409071 Lyophilized DACH1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

DACH1 stimulates shear stress-guided endothelial cell migration and coronary artery growth through the CXCL12-CXCR4 signaling axis
Chang, Raftrey, D'Amato et al
Genes Dev. (2017)
AI Summary: OriGene’s pCMV6 mouse Dachshund1 GFP-tagged plasmid (MG216327) was used for Dach1 overexpression experiments, enabling the authors to investigate how DACH1 regulates endothelial cell behavior and coronary artery growth.
Snippet: For Dach1 overexpression experiments, pCMV6 mouse Dachs- hund1 GFP-tagged (transcript variant 1, OriGene Technologies, MG216327) was purchased, and the Dach1 -coding region was subcloned into a lentiviral-ready pLJM-eGFP plasmid (Addgene, plasmid 19319)
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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