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DACH1 (NM_080759) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC221505
DACH1 (Myc-DDK-tagged)-Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $1,034.00
5 Days*
Bulk Requests & Clone Modifications

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Other products for "DACH1 or NM_080759"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DACH1
Synonyms DACH
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080759
ORF Size 2118 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080759.6
RefSeq Size 5240 bp
RefSeq ORF 2121 bp
Locus ID 1602
UniProt ID Q9UI36
Cytogenetics 13q21.33
Domains Ski_Sno
Protein Families Transcription Factors
MW 73 kDa
Summary This gene encodes a chromatin-associated protein that associates with other DNA-binding transcription factors to regulate gene expression and cell fate determination during development. The protein contains a Ski domain that is highly conserved from Drosophila to human. Expression of this gene is lost in some forms of metastatic cancer, and is correlated with poor prognosis. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2009
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"NM_080759" in other vectors (7)
SKU Description Size Price
RC221505L1 Lenti ORF clone of Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,334.00
RC221505L2 Lenti ORF clone of Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1, mGFP tagged 10 ug
$1,334.00
RC221505L3 Lenti ORF clone of Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1, Myc-DDK-tagged 10 ug
$1,334.00
RC221505L4 Lenti ORF clone of Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1, mGFP tagged 10 ug
$1,334.00
RG221505 DACH1 (tGFP-tagged) - Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1 10 ug
$789.00 $1,234.00
SC120348 DACH1 (untagged)-Human dachshund homolog 1 (Drosophila) (DACH1), transcript variant 1 10 ug
$971.00
SC327980 DACH1 (untagged)-Human dachshund homolog 1 (Drosophila) (DACH1) transcript variant 1 10 ug
$1,036.00

Plasmid Map for RC221505

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Cloning scheme for RC221505
Circular map for RC221505

file-alt Citations

Effect of DACH1 on proliferation and invasion of laryngeal squamous cell carcinoma
Zhang, Ren, Wang et al
Head Face Med (2018) 14 (1), 20 DOI: 10.1186/s13005-018-0177-1
AI Summary: In this study, OriGene products—including the Plasmid-DACH1 for DACH1 overexpression and the anti-DACH1 antibody for protein detection—were used to investigate the role of DACH1 in suppressing proliferation and invasion of laryngeal squamous cell carcinoma cells.
Snippet: The human LSCC cell line Hep-2 was purchased from The Cell Bank of Chinese Academy of Science (Shanghai, China). Plasmid-DACH1 was constructed by Origene Total protein was extracted from each group. Anti-DACH1 antibody was purchased from Origene Cached
WB IHC
Showing 1-1 of 1 papers.
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