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NIPP1 (PPP1R8) (NM_138558) Human Over-expression Lysate

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SKU
LY408577
Transient overexpression lysate of protein phosphatase 1, regulatory (inhibitor) subunit 8 (PPP1R8), transcript variant 2 (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC210575
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_138558, NP_612568
Synonyms ARD-1; ARD1; NIPP-1; NIPP1; PRO2047
Predicted MW 38.5 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_612568
Locus ID 5511
Cytogenetics 1p35.3
Protein Families WB
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Other "NIPP1" proteins (7)
SKU Description Size Price
PH310575 PPP1R8 MS Standard C13 and N15-labeled recombinant protein (NP_612568) 10 ug
$3,360.00
PH318843 PPP1R8 MS Standard C13 and N15-labeled recombinant protein (NP_054829) 10 ug
$3,360.00
LC408577 Lyophilized PPP1R8 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC415484 Lyophilized PPP1R8 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY415484 Transient overexpression lysate of protein phosphatase 1, regulatory (inhibitor) subunit 8 (PPP1R8), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP310575 Recombinant protein of human protein phosphatase 1, regulatory (inhibitor) subunit 8 (PPP1R8), transcript variant 2, 20 µg 20 ug
$867.00
TP318843 Recombinant protein of human protein phosphatase 1, regulatory (inhibitor) subunit 8 (PPP1R8), transcript variant 1, 20 µg 20 ug
$867.00

file-alt Citations

The microRNA cluster miR-183/96/182 contributes to long-term memory in a protein phosphatase 1-dependent manner
Woldemichael, Jawaid, Kremer et al
Nat Commun (2016) 7, 12594 DOI: 10.1038/ncomms12594
Cited by 64
AI Summary: OriGene’s NIPP1 plasmid was used in this study to inhibit nuclear PP1 in primary miRNA processing assays, enabling the authors to investigate the role of PP1 in regulating the miR-183/96/182 cluster during memory formation.
Snippet: In primiRNA processing assays, inhibition of nuclear PP1 was achieved by overexpression of a plasmid containing NIPP1 construct (Origene)
Showing 1-1 of 1 papers.
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