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MAP4K4 (NM_145686) Human Over-expression Lysate

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SKU
LY407892
Transient overexpression lysate of mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 2 (as WB positive control)
  $665.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC215163
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_145686, NP_663719
Synonyms FLH21957; HEL-S-31; HGK; MEKKK4; NIK
Predicted MW 150.8 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_663719
Locus ID 9448
Cytogenetics 2q11.2
Protein Families WB
Protein Pathways MAPK signaling pathway
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Documents
Resources
Other "MAP4K4" proteins (1)
SKU Description Size Price
LC407892 Lyophilized MAP4K4 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

MAP4K4 promotes epithelial-mesenchymal transition and metastasis in hepatocellular carcinoma
Feng, Pan, Wang et al
Tumour Biol. (2016)
Cited by 30
AI Summary: OriGene products contributed to this study by providing the full-length human MAP4K4 cDNA (catalog number SC309964) and the pCMV6-XL4 expression vector, which were used to overexpress MAP4K4 in hepatocellular carcinoma cells to investigate its role in EMT and metastasis.
Snippet: MAP4K4 overexpression Full-length human MAP4K4 (NM_004834) cDNA was purchased from OriGene Technologies Inc. (SC309964, Rockville, MD, USA) and subcloned into the pCMV6-XL4 ex- pression vector (OriGene Technologies Inc.). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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