MAP4K4 (NM_145686) Human Tagged ORF Clone

SKU RC215163

MAP4K4 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MAP4K4
• Synonyms: FLH21957; HEL-S-31; HGK; MEKKK4; NIK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215163 representing NM_145686; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCTGCTGGGA; TTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAAC; GGGTCAGTTGGCAGCCATCAAAGTTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATA; AATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGCTTTCATCAAAAAGAGCC; CTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGT; GAAGAACACCAAAGGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGA; CTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCAAGGGCCAGAATGTGTTGCTGACTGAGA; ATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATAC; GTTCATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTAT; GATTACAGAAGTGATCTTTGGTCTTGTGGCATTACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCT; GTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGTCAAAAAA; ATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACA; GAGCAGCTTTTGAAACATCCTTTTATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGG; ATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTATGAGTACAGTGGGAGTGA; GGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCT; ACTCTTCGCCGAGATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAAC; AGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGAGAGACAGAA; GCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGA; AGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAA; GGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTA; TATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAG; GCCATGTTACTGGAGTGCCGATGGCGGGAGATGGAGGAGCACCGGCAGGCAGAGAGGCTCCAGAGGCAGT; TGCAACAAGAACAAGCATATCTCCTGTCTCTACAGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCA; GCAGCCGCCACCACCGCAGCAGGAAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCCAAAGCCCACTAC; GAGCCTGCTGACCGAGCGCGAGAGGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCC; AGTCTAAGCAGACAGGCAGAGTATTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAA; CTCCGAGTCTGTGCATCCCGCCCTGCAGAGACCAGCGGAGCCACAGGTACAGTGGTCCCACCTGGCATCT; CTCAAGAACAATGTTTCCCCTGTCTCGCGATCCCATTCCTTCAGTGACCCTTCTCCCAAATTTGCACACC; ACCATCTTCGTTCTCAGGACCCATGTCCACCTTCCCGCAGTGAGGTGCTCAGTCAGAGCTCTGACTCTAA; GTCAGAGGCGCCTGACCCTACCCAAAAGGCTTGGTCTAGATCAGACAGTGACGAGGTGCCTCCAAGGGTT; CCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTCCCCACTGCAGGGCAGTGGGCAGC; AGAATAGCCAGGCAGGACAGAGAAACTCCACCAGCAGTATTGAGCCCAGGCTTCTGTGGGAGAGAGTGGA; GAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCT; CACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTC; CATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCC; TGCTGGCGAAGTGGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACGGCCACCTCAC; AAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGAGGACGACGATGTGGAGC; AGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCATCTCTGAATTTGAGCAA; TGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCA; TCCAAGGAGGGCACTCTAATCGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTT; CCTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGAC; ATCTGTGGTGGGATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGC; TCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATACAAGA; AGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGG; CCTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAA; ATGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACT; ATTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAAC; CGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATT; GCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCACAAATTTATGGCCTTTAAGT; CATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGT; GATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTA; CCAACACATATCCAGTGTAGCATCAAACCCCATGCAATCATCATCCTCCCCAATACAGATGGAATGGAGC; TTCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAGTTCT; ACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAG; GCCATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGAC; TAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGT; TTATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215163 representing NM_145686; Red=Cloning site Green=Tags(s); ; MANDSPAKSLVDIDLSSLRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKLEI; NMLKKYSHHRNIATYYGAFIKKSPPGHDDQLWLVMEFCGAGSITDLVKNTKGNTLKEDWIAYISREILRG; LAHLHIHHVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATY; DYRSDLWSCGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFFSFIEGCLVKNYMQRPST; EQLLKHPFIRDQPNERQVRIQLKDHIDRTRKKRGEKDETEYEYSGSEEEEEEVPEQEGEPSSIVNVPGES; TLRRDFLRLQQENKERSEALRRQQLLQEQQLREQEEYKRQLLAERQKRIEQQKEQRRRLEEQQRREREAR; RQQEREQRRREQEEKRRLEELERRRKEEEERRRAEEEKRRVEREQEYIRRQLEEEQRHLEVLQQQLLQEQ; AMLLECRWREMEEHRQAERLQRQLQQEQAYLLSLQHDHRRPHPQHSQQPPPPQQERSKPSFHAPEPKAHY; EPADRAREVEDRFRKTNHSSPEAQSKQTGRVLEPPVPSRSESFSNGNSESVHPALQRPAEPQVQWSHLAS; LKNNVSPVSRSHSFSDPSPKFAHHHLRSQDPCPPSRSEVLSQSSDSKSEAPDPTQKAWSRSDSDEVPPRV; PVRTTSRSPVLSRRDSPLQGSGQQNSQAGQRNSTSSIEPRLLWERVEKLVPRPGSGSSSGSSNSGSQPGS; HPGSQSGSGERFRVRSSSKSEGSPSQRLENAVKKPEDKKEVFRPLKPAGEVDLTALAKELRAVEDVRPPH; KVTDYSSSSEESGTTDEEDDDVEQEGADESTSGPEDTRAASSLNLSNGETESVKTMIVHDDVESEPAMTP; SKEGTLIVRQTQSASSTLQKHKSSSSFTPFIDPRLLQISPSSGTTVTSVVGFSCDGMRPEAIRQDPTRKG; SVVNVNPTNTRPQSDTPEIRKYKKRFNSEILCAALWGVNLLVGTESGLMLLDRSGQGKVYPLINRRRFQQ; MDVLEGLNVLVTISGKKDKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDLEGCVHYKVVKYERIKFLVI; ALKSSVEVYAWAPKPYHKFMAFKSFGELVHKPLLVDLTVEEGQRLKVIYGSCAGFHAVDVDSGSVYDIYL; PTHIQCSIKPHAIIILPNTDGMELLVCYEDEGVYVNTYGRITKDVVLQWGEMPTSVAYIRSNQTMGWGEK; AIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLGRTSLLSW; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_145686
• ORF Size: 3960 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145686.2, NP_663719.1
• RefSeq Size: 7495 bp
• RefSeq ORF: 3822 bp
• Locus ID: 9448
• UniProt ID: O95819
• Cytogenetics: 2q11.2
• Domains: CNH, pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway
• MW: 150.8 kDa
• Summary: The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]