MAP4K4 (NM_145686) Human Untagged Clone

SKU SC100241

MAP4K4 (untagged)-Human mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: MAP4K4
• Synonyms: FLH21957; HEL-S-31; HGK; MEKKK4; NIK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_145686, the custom clone sequence may differ by one or more nucleotides; ; ; ATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCTGCTGGGA; TTTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAAC; GGGTCAGTTGGCAGCCATCAAAGTTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATA; AATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGCTTTCATCAAAAAGAGCC; CTCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGT; GAAGAACACCAAAGGGAACACACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGA; CTGGCACATCTTCACATTCATCATGTGATTCACCGGGATATCAAGGGCCAGAATGTGTTGCTGACTGAGA; ATGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATAC; GTTCATAGGCACTCCCTACTGGATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTAT; GATTACAGAAGTGATCTTTGGTCTTGTGGCATTACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCT; GTGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAAGTCAAAAAA; ATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTACATGCAGCGGCCCTCTACA; GAGCAGCTTTTGAAACATCCTTTTATAAGGGATCAGCCAAATGAAAGGCAAGTTAGAATCCAGCTTAAGG; ATCATATAGATCGTACCAGGAAGAAGAGAGGCGAGAAAGATGAAACTGAGTATGAGTACAGTGGGAGTGA; GGAAGAAGAGGAGGAAGTGCCTGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCT; ACTCTTCGCCGAGATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAAC; AGTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGAGAGACAGAA; GCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAGGAGAGAGCGGGAAGCTAGA; AGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGAAAAGAGGCGTCTAGAGGAGTTGGAGAGAA; GGCGCAAAGAAGAAGAGGAGAGGAGACGGGCAGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTA; TATCAGGCGACAGCTAGAAGAGGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAG; GCCATGTTACTGCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGG; AAAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCGAGCGCGAGA; GGTGGAAGATAGATTTAGGAAAACTAACCACAGCTCCCCTGAAGCCCAGTCTAAGCAGACAGGCAGAGTA; TTGGAGCCACCAGTGCCTTCCCGATCAGAGTCTTTTTCCAATGGCAACTCCGAGTCTGTGCATCCCGCCC; TGCAGAGACCAGCGGAGCCACAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGA; TTCCCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGCAGTATTGAG; CCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAGCTCCTCAGGGTCCA; GCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGGCTCCGGGGAACGCTTCAGAGTGAG; ATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCGCCTGGAAAATGCAGTGAAAAAACCTGAAGATAAA; AAGGAAGTTTTCAGACCCCTCAAGCCTGCTGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAG; ATGTACGGCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGAGGA; GGACGACGATGTGGAGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGACACCAGAGCAGCGTCA; TCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGATTGTCCATGATGATGTAGAAAGTG; AGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAATCGTCCGCCAGAGTACAGTTGACCAAAAGCGTGC; CAGCCATCATGAGAGCAATGGCTTTGCCGGTCGCATTCACCTCTTGCCAGATCTCTTACAGCAAAGCCAT; TCCTCCTCCACTTCCTCCACCTCCTCCTCCCCATCCTCCAGCCAGCCGACACCCACCATGTCCCCACAGA; CACCCCAGGACAAGCTCACTGCTAATGAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTC; CTCCTCCTTTACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGACA; TCTGTGGTGGGATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTACCCGGAAAGGCT; CAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCCGGAGATTCGTAAATACAAGAA; GAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGGAGTGAATTTGCTAGTGGGTACAGAGAGTGGC; CTGATGCTGCTGGACAGAAGTGGCCAAGGGAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAA; TGGACGTACTTGAGGGCTTGAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTA; TTTGTCCTGGTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAACC; GTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATTTCTGGTGATTG; CTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCACAAATTTATGGCCTTTAAGTC; ATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCTCACTGTTGAGGAAGGCCAGAGGTTGAAAGTG; ATCTATGGATCCTGTGCTGGATTCCATGCTGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTAC; CAACACATATCCAGTGTAGCATCAAACCCCATGCAATCATCATCCTCCCCAATACAGATGGAATGGAGCT; TCTGGTGTGCTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAGTTCTA; CAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTGGGGAGAGAAGG; CCATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTCATGCACAAAAGGGCTCAAAGACT; AAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGCCTCTGTTCGGTCTGGTGGCAGCAGTCAGGTT; TATTTCATGACCTTAGGCAGGACTTCTCTTCTGAGCTGGTAG; ;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_145686 unedited; CATTTGNTATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGATATACTAAAAT; TGGAACGATACAGAGAAGATTAGCATGGCCCCTGCGCAAGGATGACACGCAAATTCGTGA; AGCGTTCCATATTTTTTTGTTATTTGTTTTTTGGTGGCAAAAAGGGAAAATGGCGAACGA; CTCCCCTGCAAAAAGTCTGGTGGACATCGACCTCTCCTCCCTGCGGGATCCTGCTGGGAT; TTTTGAGCTGGTGGAAGTGGTTGGAAATGGCACCTATGGACAAGTCTATAAGGGTCGACA; TGTTAAAACGGGTCAGTTGGCAGCCATCAAAGTTATGGATGTCACTGAGGATGAAGAGGA; AGAAATCAAACTGGAGATAAATATGCTAAAGAAATACTCTCATCACAGAAACATTGCAAC; ATATTATGGTGCTTTCATCAAAAAGAGCCCTCCAGGACATGATGACCAACTCTGGCTTGT; TATGGAGTTCTGTGGGGCTGGGTCCATTACAGACCTTGTGAAGAACACCAAAGGGAACAC; ACTCAAAGAAGACTGGATCGCTTACATCTCCAGAGAAATCCTGAGGGGACTGGCACATCT; TCACATTCATCATGTGATTCACCGGGATATCAAGGGCCAGAATGTGTTGCTGACTGAGAA; TGCAGAGGTGAAACTTGTTGACTTTGGTGTGAGTGCTCAGCTGGACAGGACTGTGGGGCG; GAGAAATACGTTCATAGGCACTCCCTACTGGATGGCTCCTGNAGTCATCGCCTGTGATGA; GAACCCAGATGCCACCTATGATTACAGAAGTGATCTTTGGTCTTTGTGCATTACAGCCAT; TGAGATGCCAGAAGGTGCTCCCCCTCTCTGNGACATGCATCCAATGAGAGCACTGNTTCT; CATTCCAGAAACCTCCTNCCCCGCTGTAGTCAAAAAATGGTCCGAGAAAGTTTTTTAGTT; TATANAAAGGGGCCCTGTGAGAATCCATGCAGNGGCCCTCTCANAACACTTTTGAACAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_145686
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145686.2, NP_663719.1
• RefSeq Size: 7495 bp
• RefSeq ORF: 3963 bp
• Locus ID: 9448
• UniProt ID: O95819
• Cytogenetics: 2q11.2
• Domains: CNH, pkinase, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway
• Summary: The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.