H4-16 (NM_175054) Human Over-expression Lysate
SKU
LY406378
Transient overexpression lysate of histone cluster 4, H4 (HIST4H4) (as WB positive control)
$436.00
5 Days*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC205064 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_175054, NP_778224 |
| Synonyms | H4/p; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C14; H4C15; HIST4H4 |
| Predicted MW | 11.2 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_778224 |
| Locus ID | 121504 |
| Cytogenetics | 12p12.3 |
| Protein Families | WB |
| Protein Pathways | Systemic lupus erythematosus |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "H4-16" proteins (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH305064 | HIST4H4 MS Standard C13 and N15-labeled recombinant protein (NP_778224) | 10 ug |
$3,360.00
|
|
| LC406378 | Lyophilized HIST4H4 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| TP305064 | Recombinant protein of human histone cluster 4, H4 (HIST4H4), 20 µg | 20 ug |
$737.00
|
|
Citations
Activation of histamine H4 receptors decreases epithelial-to-mesenchymal transition progress by inhibiting transforming growth factor-β1 signalling pathway in non-small cell lung cancer
Cai, Hu, Li et al
Eur. J. Cancer (2014)
Cai, Hu, Li et al
Eur. J. Cancer (2014)
Cited by 32
AI Summary: OriGene’s human H4 receptor cDNA was used to overexpress the H4 receptor in A549 cells, enabling the authors to investigate the role of H4 receptor activation in suppressing epithelial-to-mesenchymal transition in non-small cell lung cancer.
Snippet: control. For H 4 receptor overexpression, A549 cells were transfected with 2 μg human H 4 receptor cDNA (Origene, Rockville, MD) as described [7]. LacZ was used as control using TransIT-LT1 Reagent (Mirus, Madison, WI). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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