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H4-16 (NM_175054) Human Over-expression Lysate

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SKU
LC406378
Lyophilized HIST4H4 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC205064
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_175054, NP_778224
Synonyms H4/p; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C14; H4C15; HIST4H4
Predicted MW 11.2 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_778224
Locus ID 121504
Cytogenetics 12p12.3
Protein Families WB
Protein Pathways Systemic lupus erythematosus
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Other "H4-16" proteins (3)
SKU Description Size Price
PH305064 HIST4H4 MS Standard C13 and N15-labeled recombinant protein (NP_778224) 10 ug
$3,360.00
LY406378 Transient overexpression lysate of histone cluster 4, H4 (HIST4H4) (as WB positive control) 100 ug
$436.00
TP305064 Recombinant protein of human histone cluster 4, H4 (HIST4H4), 20 µg 20 ug
$737.00

file-alt Citations

Activation of histamine H4 receptors decreases epithelial-to-mesenchymal transition progress by inhibiting transforming growth factor-β1 signalling pathway in non-small cell lung cancer
Cai, Hu, Li et al
Eur. J. Cancer (2014)
Cited by 32
AI Summary: OriGene’s human H4 receptor cDNA was used to overexpress the H4 receptor in A549 cells, enabling the authors to investigate the role of H4 receptor activation in suppressing epithelial-to-mesenchymal transition in non-small cell lung cancer.
Snippet: control. For H 4 receptor overexpression, A549 cells were transfected with 2 μg human H 4 receptor cDNA (Origene, Rockville, MD) as described [7]. LacZ was used as control using TransIT-LT1 Reagent (Mirus, Madison, WI). ... Cite SaveSaving
Showing 1-1 of 1 papers.
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