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CREB3L2 (NM_194071) Human Over-expression Lysate

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SKU
LY405179
Transient overexpression lysate of cAMP responsive element binding protein 3-like 2 (CREB3L2) (as WB positive control)
  $436.00
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC211063
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_194071, NP_919047
Synonyms BBF2H7
Predicted MW 57.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_919047
Locus ID 64764
Cytogenetics 7q33
Protein Families WB
Protein Pathways Huntington's disease, Melanogenesis, Prostate cancer
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Other "CREB3L2" proteins (1)
SKU Description Size Price
LC405179 Lyophilized CREB3L2 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00

file-alt Citations

CREB3L2 Modulates Nerve Growth Factor-Induced Cell Differentiation
Sampieri, Funes Chabán, Di Giusto et al
Front Mol Neurosci (2021) 14, 650338 DOI: 10.3389/fnmol.2021.650338
Cited by 18
AI Summary: In this study, OriGene products—specifically the CREB3L2 cDNA clone (Cat# RC223216) and CREB3L2 shRNA plasmid (Cat# TL320991)—were used to overexpress and knock down CREB3L2 in PC12 cells, enabling the authors to investigate CREB3L2's role in modulating nerve growth factor-induced neuronal differentiation.
FigureFigureFigure
Showing 1-1 of 1 papers.
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