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CHMP2A (NM_198426) Human Over-expression Lysate

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SKU
LY404873
Transient overexpression lysate of chromatin modifying protein 2A (CHMP2A), transcript variant 2 (as WB positive control)
  $436.00
5 Days*
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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC218563
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_198426, NP_940818
Synonyms BC-2; BC2; CHMP2; VPS2; VPS2A
Predicted MW 25.1 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_940818
Locus ID 27243
Cytogenetics 19q13.43
Protein Families WB
Protein Pathways Endocytosis
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Other "CHMP2A" proteins (5)
SKU Description Size Price
PH318563 CHMP2A MS Standard C13 and N15-labeled recombinant protein (NP_940818) 10 ug
$3,360.00
LC404873 Lyophilized CHMP2A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC415258 Lyophilized CHMP2A HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY415258 Transient overexpression lysate of chromatin modifying protein 2A (CHMP2A), transcript variant 1 (as WB positive control) 100 ug
$436.00
TP318563 Recombinant protein of human chromatin modifying protein 2A (CHMP2A), transcript variant 2, 20 µg 20 ug
$867.00

file-alt Citations

CHMP2A regulates broad immune cell-mediated antitumor activity in an immunocompetent in vivo head and neck squamous cell carcinoma model
Yun, Saddawi-Konefka, Goldenson et al
J Immunother Cancer (2024) 12 (5) DOI: 10.1136/jitc-2023-007187
Cited by 6
AI Summary: OriGene's CHMP2A overexpression lysate (Cat# LC404873) was used as a control in supplemental experiments to assess CHMP2A protein levels in the study investigating immune cell-mediated antitumor activity.
Snippet: levels were detected using Immobilon Western Chemiluminescent HRP Substrate (EMD Millipore # WBKLS0500). CHMP2A overexpression 7 lysate (OL) control used in Supplemental figure 1C was purchased from Origene # LC404873. Mouse NK cell isolation Five C57Bl/6 mice were injected with 200 µg of poly I:C per injection and resuspended in sterile water (Invivogen #tlrl-picw) 1 day before isolating N
FigureFigureFigure
CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity
Bernareggi, Xie, Prager et al
Nat Commun (2022) 13 (1), 1899 DOI: 10.1038/s41467-022-29469-0
Cited by 73
AI Summary: OriGene’s CHMP2A (NM_014453) Human Tagged ORF Clone was used to generate CHMP2A-overexpressing CAL27 cells, enabling the authors to study the role of CHMP2A in tumor resistance to NK cell-mediated cytotoxicity.
Snippet: Lee (Nationwide Children’s Hospital) for expansion71. Generation of CHMP2A overexpressing Cal27 In all, 8 × 105 cells Cal27-KO were transfected with CHMP2A (NM_014453) Human Tagged ORF Clone (Origene) via nucleofection using Amaxa 2D (Lonza) and Cell Line Nucleofector Kit V (Lonza) following manufacturer’s protocol. After nucleofection cells were seeded in six-well plates and 24 h late
FigureFigureFigure
Showing 1-2 of 2 papers.
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