CHMP2A (NM_198426) Human Over-expression Lysate
SKU
LC404873
Lyophilized CHMP2A HEK293T cell transient overexpression lysate (as WB positive control)
$206.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC218563 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_198426, NP_940818 |
| Synonyms | BC-2; BC2; CHMP2; VPS2; VPS2A |
| Predicted MW | 25.1 kDa |
| Components | 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate |
| Storage | The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C. |
| Shipping | Blue Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins. |
| Reconstitution Method | The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel. The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended. After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. |
| Reference Data | |
| RefSeq | NP_940818 |
| Locus ID | 27243 |
| Cytogenetics | 19q13.43 |
| Protein Families | WB |
| Protein Pathways | Endocytosis |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "CHMP2A" proteins (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| PH318563 | CHMP2A MS Standard C13 and N15-labeled recombinant protein (NP_940818) | 10 ug |
$3,360.00
|
|
| LC415258 | Lyophilized CHMP2A HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
|
| LY404873 | Transient overexpression lysate of chromatin modifying protein 2A (CHMP2A), transcript variant 2 (as WB positive control) | 100 ug |
$436.00
|
|
| LY415258 | Transient overexpression lysate of chromatin modifying protein 2A (CHMP2A), transcript variant 1 (as WB positive control) | 100 ug |
$436.00
|
|
| TP318563 | Recombinant protein of human chromatin modifying protein 2A (CHMP2A), transcript variant 2, 20 µg | 20 ug |
$867.00
|
|
Citations
CHMP2A regulates broad immune cell-mediated antitumor activity in an immunocompetent in vivo head and neck squamous cell carcinoma model
Yun, Saddawi-Konefka, Goldenson et al
J Immunother Cancer (2024) 12 (5) DOI: 10.1136/jitc-2023-007187
Yun, Saddawi-Konefka, Goldenson et al
J Immunother Cancer (2024) 12 (5) DOI: 10.1136/jitc-2023-007187
Cited by 6
AI Summary: OriGene's CHMP2A overexpression lysate (Cat# LC404873) was used as a control in supplemental experiments to assess CHMP2A protein levels in the study investigating immune cell-mediated antitumor activity.
Snippet: levels were detected using Immobilon Western Chemiluminescent HRP Substrate (EMD Millipore # WBKLS0500). CHMP2A overexpression 7 lysate (OL) control used in Supplemental figure 1C was purchased from Origene # LC404873. Mouse NK cell isolation Five C57Bl/6 mice were injected with 200 µg of poly I:C per injection and resuspended in sterile water (Invivogen #tlrl-picw) 1 day before isolating N
CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity
Bernareggi, Xie, Prager et al
Nat Commun (2022) 13 (1), 1899 DOI: 10.1038/s41467-022-29469-0
Bernareggi, Xie, Prager et al
Nat Commun (2022) 13 (1), 1899 DOI: 10.1038/s41467-022-29469-0
Cited by 73
AI Summary: OriGene’s CHMP2A (NM_014453) Human Tagged ORF Clone was used to generate CHMP2A-overexpressing CAL27 cells, enabling the authors to study the role of CHMP2A in tumor resistance to NK cell-mediated cytotoxicity.
Snippet: Lee (Nationwide Children’s Hospital) for expansion71. Generation of CHMP2A overexpressing Cal27 In all, 8 × 105 cells Cal27-KO were transfected with CHMP2A (NM_014453) Human Tagged ORF Clone (Origene) via nucleofection using Amaxa 2D (Lonza) and Cell Line Nucleofector Kit V (Lonza) following manufacturer’s protocol. After nucleofection cells were seeded in six-well plates and 24 h late
Showing 1-2 of 2 papers.
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