LRRK2 (NM_198578) Human Over-expression Lysate
SKU
LY404831
Transient overexpression lysate of leucine-rich repeat kinase 2 (LRRK2) (as WB positive control)
$665.00
2 Weeks*
Specifications
| Product Data | |
| Species | Human |
|---|---|
| Expression Host | HEK293T |
| Expression cDNA Clone or AA Sequence | TrueORF Clone RC216373 |
| Tag | C-Myc/DDK |
| Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
| ACCN | NM_198578, NP_940980 |
| Synonyms | AURA17; DARDARIN; PARK8; RIPK7; ROCO2 |
| Predicted MW | 285.9 kDa |
| Components | 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer |
| Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
| Shipping | Dry Ice |
| Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
| Reference Data | |
| RefSeq | NP_940980 |
| Locus ID | 120892 |
| Cytogenetics | 12q12 |
| Protein Families | WB |
| Protein Pathways | Parkinson's disease |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
| Recombinant Protein Resources |
Other "LRRK2" proteins (1)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| LC404831 | Lyophilized LRRK2 HEK293T cell transient overexpression lysate (as WB positive control) | 20 ug |
$206.00
|
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Citations
Coordinate Regulation of Neurite Outgrowth by LRRK2 and Its Interactor, Rab5
Heo, Kim, Seol
Exp Neurobiol (2010) 19 (2), 97-105 DOI: 10.5607/en.2010.19.2.97
Heo, Kim, Seol
Exp Neurobiol (2010) 19 (2), 97-105 DOI: 10.5607/en.2010.19.2.97
Cited by 49
AI Summary: In this study, OriGene’s TrueORF cDNA clones for human LRRK2 and Rab5b were used to over-express these proteins in neuronal cells, enabling the authors to investigate how LRRK2 and Rab5b coordinate the regulation of neurite outgrowth.
The LINK-A lncRNA activates normoxic HIF1 [alpha] signalling in triple-negative breast cancer
Lin, Li, Xing et al
Nature cell biology (2016)
Lin, Li, Xing et al
Nature cell biology (2016)
Snippet: Yang and colleagues identify a lncRNA that controls HIF1[alpha] signalling to stabilize HIF1[alpha] under normoxic conditions, and promotes breast cancer tumorigenesis. Cite SaveSaving
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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