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LRRK2 (NM_198578) Human Over-expression Lysate

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SKU
LC404831
Lyophilized LRRK2 HEK293T cell transient overexpression lysate (as WB positive control)
Size:
20 ug 100 ug
Formulation:
Lyophilized Liquid
  $206.00
2 Weeks*
Bulk/Customize

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Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC216373
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_198578, NP_940980
Synonyms AURA17; DARDARIN; PARK8; RIPK7; ROCO2
Predicted MW 285.9 kDa
Components 1 vial of 20 ug lyophilized total protein derived from gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature, however upon receipt of the sample store at -20°C. Lysate samples are stable for 12 months from the date of receipt when stored at -20°C in their original lyophilized form.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Shipping Blue Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution Method The 20 µg lyophilized sample can be reconstituted with deionized water, or directly into 1x SDS-PAGE running buffer (with reducing agent) if the sample is being prepared directly to be run on a gel.  The lyophilized 20 ug product is derived from a liquid solution at 1 mg/ml of total lysate protein, thus reconstituting back to 1 mg/ml using 20 µl is recommended.  After reconstitution, avoid repeat freeze thaw cycles and store at -80°C.
Reference Data
RefSeq NP_940980
Locus ID 120892
Cytogenetics 12q12
Protein Families WB
Protein Pathways Parkinson's disease
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Documents
Resources
Other "LRRK2" proteins (1)
SKU Description Size Price
LY404831 Transient overexpression lysate of leucine-rich repeat kinase 2 (LRRK2) (as WB positive control) 100 ug
$665.00

file-alt Citations

Coordinate Regulation of Neurite Outgrowth by LRRK2 and Its Interactor, Rab5
Heo, Kim, Seol
Exp Neurobiol (2010) 19 (2), 97-105 DOI: 10.5607/en.2010.19.2.97
Cited by 49
AI Summary: In this study, OriGene’s TrueORF cDNA clones for human LRRK2 and Rab5b were used to over-express these proteins in neuronal cells, enabling the authors to investigate how LRRK2 and Rab5b coordinate the regulation of neurite outgrowth.
FigureFigureFigure
The LINK-A lncRNA activates normoxic HIF1 [alpha] signalling in triple-negative breast cancer
Lin, Li, Xing et al
Nature cell biology (2016)
Snippet: Yang and colleagues identify a lncRNA that controls HIF1[alpha] signalling to stabilize HIF1[alpha] under normoxic conditions, and promotes breast cancer tumorigenesis. Cite SaveSaving
Showing 1-2 of 2 papers.
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