OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

SMURF1 (NM_181349) Human Over-expression Lysate

Be the first to review this product
SKU
LY403618
Transient overexpression lysate of SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2 (as WB positive control)
  $665.00
5 Days*
Bulk/Customize

Frequently Bought Together (3)

TA350761 Rabbit Polyclonal Anti-SMURF1 Antibody
100 ul
$205.00
TA811000S beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control 30 ul
$200.00
TA592569 DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
100 ul
$519.00

Other products for "SMURF1 or NM_181349"

Specifications
Specifications
Product Data
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC219536
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
ACCN NM_181349, NP_851994
Predicted MW 83.3 kDa
Components 1 vial of 100 µg total protein derived from gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 1x SDS-PAGE running buffer (with reducing agent). Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C.
Shipping Dry Ice
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.).
Reference Data
RefSeq NP_851994
Locus ID 57154
Cytogenetics 7q22.1
Protein Families WB
Protein Pathways Endocytosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
Other "SMURF1" proteins (5)
SKU Description Size Price
PH319536 SMURF1 MS Standard C13 and N15-labeled recombinant protein (NP_851994) 10 ug
$3,360.00
LC403618 Lyophilized SMURF1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LC412480 Lyophilized SMURF1 HEK293T cell transient overexpression lysate (as WB positive control) 20 ug
$206.00
LY412480 Transient overexpression lysate of SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 1 (as WB positive control) 100 ug
$665.00
TP319536 Recombinant protein of human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, 20 µg 20 ug
$867.00

file-alt Citations

SMURF1 and SMURF2 in Progenitor Cells from Articular Cartilage and Meniscus during Late-Stage Osteoarthritis
Schminke, Kauffmann, Schubert et al
Cartilage (2020)
Cited by 9
AI Summary: In this study, OriGene’s SMURF1 siRNA (SR311389BL) was used to specifically knock down SMURF1 expression in progenitor cells, enabling the researchers to investigate its role in regulating key transcription factors involved in osteoarthritis.
Snippet: ObjectiveThe aim of this study was to investigate the roles of SMURF1 and SMURF2 in progenitor cells from the human knee in late-stage osteoarthritis (OA).DesignWe applied immunohistochemistry, imm...;For transfection of CPCs and MPCs, we used Human-MSC Nucleofactor Kit (VPE-1001;Lonza). siRNA was obtained commercially for SMURF1 (SR311389BL;OriGene) and SMURF2 (SI00134295;Qiagen). We harvested 5 × 105 CPCs and MPCs at passage 4 and resuspended the cells in 100 µL of human chondrocyte nucleofector solution (VPF1001;Lonza) with 10 µL siRNA of SMURF1 or SMURF2 at 0.2 nmol
Bardoxolone ameliorates TGF-β1-associated renal fibrosis through Nrf2/Smad7 elevation
Song, Lee, Ryoo et al
Free Radic. Biol. Med. (2019) 138, 33-42 DOI: 10.1016/j.freeradbiomed.2019.04.033
AI Summary: OriGene’s pCMV6-Smurf1 plasmid was used to overexpress Smurf1 in cells, enabling the authors to investigate the role of Smurf1 in regulating Smad7 levels and its impact on TGF-β1-associated renal fibrosis.
Snippet: Corporation (Daejeon, Korea). For Smurf1 overexpression, pCMV6-Smurf1 (Origene, Rockville, MD, USA) was transfected in cells using Lipofectamine 2000. 2.5. Total RNA extraction and real-time PCR. Total RNAs were isolated
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products